Productive Infection of Mouse Mammary Glands and Human Mammary Epithelial Cells by Zika Virus

被引:6
|
作者
Hubert, Mathieu [1 ,2 ]
Chiche, Aurelie [3 ]
Legros, Vincent [1 ,2 ]
Jeannin, Patricia [1 ]
Montange, Thomas [1 ]
Gessain, Antoine [1 ]
Ceccaldi, Pierre-Emmanuel [1 ,2 ]
Vidy, Aurore [1 ,2 ]
机构
[1] Inst Pasteur, UMR Ctr Natl Rech Sci 3569, Dept Virol, Unite Epidemiol & Physiopathol Virus Oncogenes, F-75015 Paris, France
[2] Univ Paris, F-75013 Paris, France
[3] Inst Pasteur, Dept Biol Dev & Cellules Souches, Grp 5 Ans Plasticite Cellulaire & Modelisat Malad, F-75015 Paris, France
来源
VIRUSES-BASEL | 2019年 / 11卷 / 10期
关键词
Zika virus; dissemination; mammary glands; tropism; primary cells; luminal cells; myoepithelial cells; SEXUAL TRANSMISSION; PROLONGED DETECTION; VAGINAL SECRETIONS; MILK; SEMEN; RNA;
D O I
10.3390/v11100950
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Zika virus (ZIKV) belongs to the large category of arboviruses. Surprisingly, several human-to-human transmissions of ZIKV have been notified, either following sexual intercourse or from the mother to fetus during pregnancy. Importantly, high viral loads have been detected in the human breast milk of infected mothers, and the existence of breastfeeding as a new mode of mother-to-child transmission of ZIKV was recently hypothesized. However, the maternal origin of infectious particles in breast milk is currently unknown. Here, we show that ZIKV disseminates to the mammary glands of infected mice after both systemic and local exposure with differential kinetics. Ex vivo, we demonstrate that primary human mammary epithelial cells were sensitive and permissive to ZIKV infection in this study. Moreover, by using in vitro models, we prove that mammary luminal- and myoepithelial-phenotype cell lines are both able to produce important virus progeny after ZIKV exposure. Our data suggest that the dissemination of ZIKV to the mammary glands and subsequent infection of the mammary epithelium could be one mechanism of viral excretion in human breast milk.
引用
收藏
页数:13
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