RETRACTED: H3K27ac-induced lncRNA PAXIP1-AS1 promotes cell proliferation, migration, EMT and apoptosis in ovarian cancer by targeting miR-6744-5p/PCBP2 axis (Retracted Article)

被引：22
作者：
Ma, Yimin [1 ]
Zheng, Wei [2 ]
机构：
[1] Lihuili Hosp, Dept Gynecol, Ningbo Med Ctr, Ningbo 315040, Zhejiang, Peoples R China
[2] Xian Mil Ind Hosp, Dept Gynecol, Xian 710065, Shaanxi, Peoples R China
来源：
JOURNAL OF OVARIAN RESEARCH | 2021年 / 14卷 / 01期
关键词：
PAXIP1-AS1; ceRNA; Ovarian cancer; H3K27 ACETYLATION PROMOTES; BREAST-CANCER; RNA; PROGRESSION; ACTIVATION; EXPRESSION; INVASION;
D O I：
10.1186/s13048-021-00822-z
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We aimed to explore role of lncRNA PAX-interacting protein 1-antisense RNA1 (PAXIP1-AS1) in ovarian cancer (OC). RT-qPCR analysis identified upregulation of PAXIP1-AS1 in OC cell lines. Functionally, PAXIP1-AS1 knockdown inhibited cell proliferation, accelerated cell apoptosis, and suppressed cell migration and epithelial-mesenchymal transition (EMT) process. Upregulation of PAXIP1-AS1 was induced by CBP-mediated H3K27 acetylation (H3K27ac) via bioinformatic analysis and ChIP assay. Furthermore, PAXIP1-AS1 served as a competing endogenous RNA (ceRNA) to regulate PCBP2 expression by sponging microRNA-6744-5p (miR-6744-5p). Restoration experiments showed that overexpressed PCBP2 rescued effects of silenced PAXIP1-AS1 on cell proliferation, apoptosis, migration and EMT. Overall, lncRNA PAXIP1-AS1 activated by H3K27ac functioned as a tumor promoter in OC via mediating miR-6744-5p/PCBP2 axis, which provided promising insight into exploration on OC therapy.
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页数：10
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共 37 条
[1]   Network biology:: Understanding the cell's functional organization [J].
Barabási, AL ;
Oltvai, ZN .
NATURE REVIEWS GENETICS, 2004, 5 (02) :101-U15
[2]   RNA Binding to CBP Stimulates Histone Acetylation and Transcription [J].
Bose, Daniel A. ;
Donahue, Greg ;
Reinberg, Danny ;
Shiekhattar, Ramin ;
Bonasio, Roberto ;
Berger, Shelley L. .
CELL, 2017, 168 (1-2) :135-+
[3]   Poly(rC) binding protein 2 (PCBP2) promotes the viability of human gastric cancer cells by regulating CDK2 [J].
Chen, Changyu ;
Lei, Jun ;
Zheng, Qiang ;
Tan, Sheng ;
Ding, Keshuo ;
Yu, Changjun .
FEBS OPEN BIO, 2018, 8 (05) :764-773
[4]   lncRNA PLAC2 activated by H3K27 acetylation promotes cell proliferation and invasion via the activation of Wnt/β-catenin pathway in oral squamous cell carcinoma [J].
Chen, Fubo ;
Qi, Shengcai ;
Zhang, Xu ;
Wu, Jinjin ;
Yang, Xi ;
Wang, Raorao .
INTERNATIONAL JOURNAL OF ONCOLOGY, 2019, 54 (04) :1183-1194
[5]   RETRACTED: LncRNA HAND2-AS1 exerts anti-oncogenic effects on ovarian cancer via restoration of BCL2L11 as a sponge of microRNA-340-5p (Retracted Article) [J].
Chen, Jun ;
Lin, Yang ;
Jia, Yan ;
Xu, Tianmin ;
Wu, Fuju ;
Jin, Yuemei .
JOURNAL OF CELLULAR PHYSIOLOGY, 2019, 234 (12) :23421-23436
[6]   SP1-induced lncRNA-ZFAS1 contributes to colorectal cancer progression via the miR-150-5p/VEGFA axis [J].
Chen, Xiaoxiang ;
Zeng, Kaixuan ;
Xu, Mu ;
Hu, Xiuxiu ;
Lui, Xiangxiang ;
Xu, Tao ;
He, Bangshun ;
Pan, Yuqin ;
Sun, Huiling ;
Wang, Shukui .
CELL DEATH & DISEASE, 2018, 9
[7]   LncRNA KCNQ1OT1 regulates the invasion and migration of hepatocellular carcinoma by acting on S1PR1 through miR-149 [J].
Cheng, Ji-Lun ;
Li, Du-Juan ;
Lv, Ming-Yang ;
Pei, Yi-Jin ;
Zhang, Xiu-Juan ;
Li, Lin ;
Liu, Xiang-Yu ;
Fan, Ai-Hui .
CANCER GENE THERAPY, 2021, 28 (7-8) :850-863
[8]   Histone H3K27ac separates active from poised enhancers and predicts developmental state [J].
Creyghton, Menno P. ;
Cheng, Albert W. ;
Welstead, G. Grant ;
Kooistra, Tristan ;
Carey, Bryce W. ;
Steine, Eveline J. ;
Hanna, Jacob ;
Lodato, Michael A. ;
Frampton, Garrett M. ;
Sharp, Phillip A. ;
Boyer, Laurie A. ;
Young, Richard A. ;
Jaenisch, Rudolf .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (50) :21931-21936
[9]   LncRNA GHET1 activated by H3K27 acetylation promotes cell tumorigenesis through regulating ATF1 in hepatocellular carcinoma [J].
Ding, Gangqiang ;
Li, Wei ;
Liu, Junping ;
Zeng, Yanli ;
Mao, Chongshan ;
Kang, Yi ;
Shang, Jia .
BIOMEDICINE & PHARMACOTHERAPY, 2017, 94 :326-331
[10]   LINC-PINTsuppresses tumour cell proliferation, migration and invasion through targetingmiR-374a-5p in ovarian cancer [J].
Hao, Ting ;
Huang, Shu ;
Han, Fangzheng .
CELL BIOCHEMISTRY AND FUNCTION, 2020, 38 (08) :1089-1099
1234