Pisciglobus halotolerans gen. nov., sp nov., isolated from fish sauce

Two strains of Gram-stain-positive, catalase-negative, tetrad-forming cocci, C01(T) and C02, were isolated in Thailand from fish sauce. They were facultatively anaerobic, non-motile and non-spore-forming bacteria. These strains produced L-lactic acid from glucose. They grew at pH 5.0-9.0, at 15-40 degrees C and in the presence of 10% (w/v) NaCl. The dominant fatty acid was C-18:1 omega 9c. The DNA G + C contents of strains C01(T) and C02 were 38.6 and 38.7 mol%, respectively. Strain CO1T was related most closely to Desemzia incerta DSM 20581(T), with a 16S rRNA gene sequence similarity of 96.9 % The strains could be distinguished clearly from D. incerta DSM 205811 based on cell morphology, physiological and biochemical characteristics and low levels of DNA DNA relatedness. On the basis of the data presented, strains C01(T) and C02 are considered to represent a novel species of a new genus in the Bacillus Lactobacillus cluster, for which the name Pisciglobus halotolerans gen. nov., sp. nov. is proposed. The type strain of Pisciglobus halotolerans is CO1(T) (=KCTC 13150(T) =TISTR 1958(T) =PCU 316(T)).