Mapping of a Region of the PA-X Protein of Influenza A Virus That Is Important for Its Shutoff Activity

被引：50

作者：

Oishi, Kohei ^{[1
]}

Yamayoshi, Seiya ^{[1
]}

Kawaoka, Yoshihiro ^{[1
,2
,3
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Dept Microbiol & Immunol, Div Virol, Tokyo, Japan

[2] Univ Wisconsin, Sch Vet Med, Dept Pathobiol Sci, Madison, WI 53706 USA

[3] Univ Tokyo, Inst Med Sci, Int Res Ctr Infect Dis, Dept Special Pathogens, Tokyo, Japan

来源：

JOURNAL OF VIROLOGY | 2015年 / 89卷 / 16期

基金：

日本科学技术振兴机构;

关键词：

HOST RESPONSE; SEGMENT; 3; ENDONUCLEASE; SUPPRESSION;

D O I：

10.1128/JVI.01132-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Influenza A virus PA-X comprises an N-terminal PA endonuclease domain and a C-terminal PA-X-specific domain. PA-X reduces host and viral mRNA accumulation via its endonuclease function. Here, we found that the N-terminal 15 amino acids, particularly six basic amino acids, in the C-terminal PA-X-specific region are important for PA-X shutoff activity. These six basic amino acids enabled a PA deletion mutant to suppress protein expression at a level comparable to that of wild-type PA-X.

引用

页码：8661 / 8665

页数：5

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