Non-thermal dielectric barrier discharge plasma induces angiogenesis through reactive oxygen species

被引:160
作者
Arjunan, Krishna Priya [1 ]
Friedman, Gary
Fridman, Alexander
Clyne, Alisa Morss [1 ]
机构
[1] Drexel Univ, Sch Biomed Engn Sci & Hlth Syst, Philadelphia, PA 19104 USA
关键词
vascularization; wound healing; angiogenesis; non-thermal plasma; reactive oxygen species; fibroblast growth factor-2 release; FIBROBLAST-GROWTH-FACTOR; MICROVASCULAR ENDOTHELIAL-CELLS; ATMOSPHERIC-PRESSURE PLASMA; FACTOR GENE-EXPRESSION; IN-VIVO ANGIOGENESIS; SMOOTH-MUSCLE-CELLS; HYDROGEN-PEROXIDE; TRANSCRIPTIONAL REGULATION; SIGNALING PATHWAYS; BLOOD-COAGULATION;
D O I
10.1098/rsif.2011.0220
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Vascularization plays a key role in processes such as wound healing and tissue engineering. Non-thermal plasma, which primarily produces reactive oxygen species (ROS), has recently emerged as an efficient tool in medical applications including blood coagulation, sterilization and malignant cell apoptosis. Liquids and porcine aortic endothelial cells were treated with a non-thermal dielectric barrier discharge plasma in vitro. Plasma treatment of phosphate-buffered saline (PBS) and serum-free medium increased ROS concentration in a dose-dependent manner, with a higher concentration observed in serum-free medium compared with PBS. Species concentration inside cells peaked 1 h after treatment, followed by a decrease 3 h post treatment. Endothelial cells treated with a plasma dose of 4.2 J cm(-2) had 1.7 times more cells than untreated samples 5 days after plasma treatment. The 4.2 J cm(-2) plasma dose increased two-dimensional migration distance by 40 per cent compared with untreated control, while the number of cells that migrated through a three-dimensional collagen gel increased by 15 per cent. Tube formation was also enhanced by plasma treatment, with tube lengths in plasma-treated samples measuring 2.6 times longer than control samples. A fibroblast growth factor-2 (FGF-2) neutralizing antibody and ROS scavengers abrogated these angiogenic effects. These data indicate that plasma enhanced proliferation, migration and tube formation is due to FGF-2 release induced by plasma-produced ROS. Non-thermal plasma may be used as a potential tool for applying ROS in precise doses to enhance vascularization.
引用
收藏
页码:147 / 157
页数:11
相关论文
共 113 条
[1]   Visfatin induces human endothelial VEGF and MMP-2/9 production via MAPK and PI3K/Akt signalling pathways: novel insights into visfatin-induced angiogenesis [J].
Adya, Raghu ;
Tan, Bee K. ;
Punn, Anu ;
Chen, Jing ;
Randeva, Harpal S. .
CARDIOVASCULAR RESEARCH, 2008, 78 (02) :356-365
[2]   Growth factor-induced therapeutic angiogenesis in the heart: protein therapy [J].
Annex, BH ;
Simons, M .
CARDIOVASCULAR RESEARCH, 2005, 65 (03) :649-655
[3]   Heating Effect of Dielectric Barrier Discharges for Direct Medical Treatment [J].
Ayan, Halim ;
Fridman, Gregory ;
Staack, David ;
Gutsol, Alexander F. ;
Vasilets, Victor N. ;
Fridman, Alexander A. ;
Friedman, Gary .
IEEE TRANSACTIONS ON PLASMA SCIENCE, 2009, 37 (01) :113-120
[4]  
Bibinov N., 2011, Biomedical Engineering, Trends in Materials Science
[5]   Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes [J].
Bienert, Gerd P. ;
Moller, Anders L. B. ;
Kristiansen, Kim A. ;
Schulz, Alexander ;
Moller, Ian M. ;
Schjoerring, Jan K. ;
Jahn, Thomas P. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (02) :1183-1192
[6]   Membrane transport of hydrogen peroxide [J].
Bienert, Gerd P. ;
Schjoerring, Jan K. ;
Jahn, Thomas P. .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2006, 1758 (08) :994-1003
[7]   Biological roles of fibroblast growth factor-2 [J].
Bikfalvi, A ;
Klein, S ;
Pintucci, G ;
Rifkin, DB .
ENDOCRINE REVIEWS, 1997, 18 (01) :26-45
[8]   Regulation of fibroblast growth factor-2 expression in pulmonary arterial smooth muscle cells involves increased reactive oxygen species generation [J].
Black, Stephen M. ;
DeVol, Jennifer M. ;
Wedgwood, Stephen .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2008, 294 (01) :C345-C354
[9]   EXPERIMENTAL AND CLINICAL OBSERVATIONS OF THE EFFECTS OF CYTO-TOXIC CHEMOTHERAPEUTIC DRUGS ON WOUND-HEALING [J].
BLAND, KI ;
PALIN, WE ;
VONFRAUNHOFER, JA ;
MORRIS, RR ;
ADCOCK, RA ;
TOBIN, GR .
ANNALS OF SURGERY, 1984, 199 (06) :782-790
[10]   RETRACTED: The future of therapeutic myocardial angiogenesis (Retracted Article) [J].
Boodhwani, Munir ;
Sodha, Neel R. ;
Laham, Roger J. ;
Sellke, Frank W. .
SHOCK, 2006, 26 (04) :332-341