String (Cdc25) regulates stem cell maintenance, proliferation and aging in Drosophila testis

被引：38
作者：
Inaba, Mayu [1 ,2 ]
Yuan, Hebao [1 ,2 ]
Yamashita, Yukiko M. [1 ,2 ,3 ]
机构：
[1] Univ Michigan, Ctr Stem Cell Biol, Inst Life Sci, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Cell & Dev Biol, Sch Med, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Cellular & Mol Biol Program, Ann Arbor, MI 48109 USA
来源：
DEVELOPMENT | 2011年 / 138卷 / 23期
基金：
美国国家卫生研究院;
关键词：
Cdc25; String; Drosophila; Stem cell; SELF-RENEWAL; GERMLINE; DIVISION; NICHE; SPERMATOGENESIS; DIFFERENTIATION; PHOSPHATASES; CENTROSOME; CANCER;
D O I：
10.1242/dev.072579
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Tight regulation of stem cell proliferation is fundamental to tissue homeostasis, aging and tumor suppression. Although stem cells are characterized by their high potential to proliferate throughout the life of the organism, the mechanisms that regulate the cell cycle of stem cells remain poorly understood. Here, we show that the Cdc25 homolog String (Stg) is a crucial regulator of germline stem cells (GSCs) and cyst stem cells (CySCs) in Drosophila testis. Through knockdown and overexpression experiments, we show that Stg is required for stem cell maintenance and that a decline in its expression during aging is a critical determinant of age-associated decline in stem cell function. Furthermore, we show that restoration of Stg expression reverses the age-associated decline in stem cell function but leads to late-onset tumors. We propose that Stg/ Cdc25 is a crucial regulator of stem cell function during tissue homeostasis and aging.
引用
收藏
页码：5079 / 5086
页数：8
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