Oxidation of a laccase mediator ABTS as studied by ESI-FTICR mass spectrometry

被引：18

作者：

Marjasvaara, Asse ^{[1
]}

Janis, Janne ^{[1
]}

Vainiotalo, Pirjo ^{[1
]}

机构：

[1] Univ Joensuu, Dept Chem, FI-80101 Joensuu, Finland

来源：

JOURNAL OF MASS SPECTROMETRY | 2008年 / 43卷 / 04期

关键词：

laccase; nanoelectrospray ionization; mass spectrometry; ABTS; oxidation; collision-induced dissociation;

D O I：

10.1002/jms.1332

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The oxidation reaction of a laccase mediator ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) was studied by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICRMS). Oxidation products of ABTS were measured after reaction times that varied from a few minutes up to several days and both positive and negative ionization modes were employed. Exact mass measurements and collision-induced dissociation (CID) experiments were used to characterize the structures of the ions formed. After reacting with Trametes versicolor laccase (TvL), the radical cation form of ABTS was the main product observed by the positive ionization mode. Negative ionization mode experiments revealed that a degradation product from ABTS was formed. Copyright (C) 2007 John Wiley & Sons, Ltd.

引用

页码：470 / 477

页数：8

共 27 条

[1] Laccase 2 is the phenoloxidase gene required for beetle cuticle tanning [J].

Arakane, Y ;

Muthukrishnan, S ;

Beeman, RW ;

Kanost, MR ;

Kramer, KJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (32) :11337-11342

[2] Promoting laccase activity towards non-phenolic substrates: a mechanistic investigation with some laccase-mediator systems [J].

Baiocco, P ;

Barreca, AM ;

Fabbrini, M ;

Galli, C ;

Gentili, P .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2003, 1 (01) :191-197

[3] A LACCASE ASSOCIATED WITH LIGNIFICATION IN LOBLOLLY-PINE XYLEM [J].

BAO, W ;

OMALLEY, DM ;

WHETTEN, R ;

SEDEROFF, RR .

SCIENCE, 1993, 260 (5108) :672-674

[4] OXIDATION OF NONPHENOLIC SUBSTRATES - AN EXPANDED ROLE FOR LACCASE IN LIGNIN BIODEGRADATION [J].

BOURBONNAIS, R ;

PAICE, MG .

FEBS LETTERS, 1990, 267 (01) :99-102

[5] Kinetics of oxidation of benzyl alcohols by the dication and radical cation of ABTS. Comparison with laccase-ABTS oxidations: an apparent paradox [J].

Branchi, B ;

Galli, C ;

Gentili, P .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2005, 3 (14) :2604-2614

[6] On the mechanism of the laccase-mediator system in the oxidation of lignin [J].

Crestini, C ;

Jurasek, L ;

Argyropoulos, DS .

CHEMISTRY-A EUROPEAN JOURNAL, 2003, 9 (21) :5371-5378

[7]

d'Acunzo F, 2002, EUR J ORG CHEM, V2002, P4195

[8] Mass selection of ions in a Fourier transform ion cyclotron resonance trap using correlated harmonic excitation fields (CHEF) [J].

de Koning, LJ ;

Nibbering, NMM ;

van Orden, SL ;

Laukien, FH .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 1997, 165 :209-219

[9] Substrate and doxygen binding to the endospore coat laccase from Bacillus subtilis [J].

Enguita, FJ ;

Marçal, D ;

Martins, LO ;

Grenha, R ;

Henriques, AO ;

Lindley, PF ;

Carrondo, MA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (22) :23472-23476

[10] Comparing the catalytic efficiency of some mediators of laccase [J].

Fabbrini, M ;

Galli, C ;

Gentili, P .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2002, 16 (5-6) :231-240

← 1 2 3 →