Discovery and functional characterization of microRNAs and their potential roles for gonadal development in spotted knifejaw, Oplegnathus punctatus

被引:15
作者
Du, Xinxin [1 ]
Liu, Xiaobing [1 ]
Zhang, Kai [1 ]
Liu, Yuxiang [1 ]
Cheng, Jie [1 ]
Zhang, Quanqi [1 ,2 ]
机构
[1] Ocean Univ China, Coll Marine Life Sci, Key Lab Marine Genet & Breeding, Minist Educ, Qingdao, Shandong, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS | 2018年 / 28卷
基金
中国国家自然科学基金;
关键词
Spotted knifejaw; microRNA; Gonadal development; Gametogenesis; Post-transcriptional regulation; SEX DETERMINATION; PIWI PROTEINS; STEM-CELLS; EXPRESSION; BIOGENESIS; GENE; IDENTIFICATION; TRANSCRIPTOME; MEDAKA; ENDOCYTOSIS;
D O I
10.1016/j.cbd.2018.05.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The spotted knifejaw (Oplegnathus punctatus) is a newly emerging economical fishery species in China. Studies focused on the regulation of gonadal development and gametogenesis of spotted knifejaw are still insufficient. As a key post-transcriptional regulator, miRNAs have been shown to play important roles in development and reproduction systems. In this study, small RNA deep sequencing in ovary and testis of spotted knifejaw were performed to screen miRNA expression patterns. After sequencing and bioinformatics analysis, a total of 247 conserved known miRNAs and 41 novel miRNAs were identified in spotted knifejaw gonads for the first time. In addition, 36 miRNAs were differentially expressed between testis and ovary. The putative target genes of differentially expressed (DE) miRNAs were significantly enriched in several pathways related to sexual differentiation and gonadal development, such as steroid hormone biosynthesis. Sequencing data was validated through qRT-PCR analysis of selected DE miRNAs. Dual-luciferase reporter analyses of filtered miRNA-target gene pairs confirmed that opu-miR-27b-3p targeted in piwi2 and mov10l1 3' UTRs and down-regulated their expressions in spotted knifejaw. The notion that mov10l1 and piwi2 enhance germ cells proliferation and regulate gonadal development and gametogenesis suggests that opu-miR-27b-3p may attenuated this process in the gonads of spotted knifejaw. These findings provided insights into regulatory roles of gonadal miRNAs and supplied fundamental resources for further studies on miRNA-mediated post-transcriptional regulation in reproductive system of spotted knifejaw.
引用
收藏
页码:1 / 8
页数:8
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