Long-term operation of microbial electrosynthesis cell reducing CO2 to multi-carbon chemicals with a mixed culture avoiding methanogenesis

被引:139
作者
Bajracharya, Suman [1 ,2 ]
Yuliasni, Rustiana [2 ]
Vanbroekhoven, Karolien [1 ]
Buisman, Cees J. N. [2 ]
Strik, David P. B. T. B. [2 ]
Pant, Deepak [1 ]
机构
[1] Flemish Inst Technol Res VITO, Separat & Convers Technol, Mol, Belgium
[2] Wageningen Univ, Subdept Environm Technol, Wageningen, Netherlands
关键词
Microbial electrosynthesis; CO2; reduction; Biocathode; Wood-Ljungdahl pathway; Autotrophic bioproduction; CARBON-DIOXIDE REDUCTION; BIOELECTROCHEMICAL SYSTEMS; FUEL-CELLS; ACETATE; CATHODE; ETHANOL; GAS; BIOCATHODES; ELECTRON; GROWTH;
D O I
10.1016/j.bioelechem.2016.09.001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In microbial electrosynthesis (IVIES), CO2 can be reduced preferablyto multi-carbon chemicals by a biocathodebased process which uses electrochemically active bacteria as catalysts. A mixed anaerobic consortium from biological origin typically produces methane from CO2 reduction which circumvents production of multi-carbon compounds. This study aimed to develop a stable and robust CO2 reducing biocathode from a mixed culture inoculum avoiding the methane generation. An effective approach was demonstrated based on (i) an enrichment procedure involving inoculum pre-treatment and several culture transfers in H-2:CO2 media, (ii) a transfer from heterotrophic to autotrophic growth and (iii) a sequentialbatch operation. Biomass growth and gradual acclimation to CO2 electro-reduction accomplished a maximum acetate production rate of 400 mg Urlatibolyte (L-catholyte(-1) d(-1) at -1V (vs. Ag/AgCl). Methane was never detected in more than 300 days of operation. Accumulation of acetate up to 7-10 g L-1 was repeatedly attained by supplying (80:20) CO2:N-2 mixture at 0.9 to 1 V (vs. Ag/AgCl). In addition, ethanol and butyrate were also produced from CO2 reduction. Thus, a robust CO2 reducing biocathode can be developed from a mixed culture avoiding methane generation by adopting the specific culture enrichment and operation procedures without the direct addition of chemical inhibitor. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:26 / 34
页数:9
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