Sodium Fluoride Affects Proliferation and Apoptosis Through Insulin-Like Growth Factor I Receptor in Primary Cultured Mouse Osteoblasts

被引:16
作者
Ren, Gaixian [1 ]
Ferreri, Miro [1 ]
Wang, Zhi [1 ]
Su, Yong [1 ]
Han, Bo [1 ]
Su, Jingliang [1 ]
机构
[1] China Agr Univ, Coll Vet Med, Beijing 100193, Peoples R China
关键词
Osteoblast; Sodium fluoride; IGF-IR; Proliferation; Apoptosis; FACTOR-BETA; EXPRESSION; BONE; SURVIVAL; CELLS; VITRO;
D O I
10.1007/s12011-011-9059-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Insulin-like growth factor-I receptor (IGF-IR) is important for maintaining proliferation and apoptosis in osteoblasts. However, the details about the effect of sodium fluoride supplementation on primary osteoblast cultures on mediating IGF-IR expression are still not well-known. In this study, we used mouse osteoblasts to examine the impact of sodium fluoride on the proliferation and apoptosis; the cells were treated with IGF-IR small interfering RNA (or left untreated) and subsequently divided into a control group and six experimental groups, which were exposed to different concentrations of NaF (10(-6), 10(-5), 10(-4), 10(-3), 5 x 10(-3), and 10(-2) mol/L) for analysis at 48 h. In particular, we examined cell proliferation, apoptosis, IGF-IR messenger RNA, and protein expression levels of the various cell groups. In summary, our findings suggest that the administration of NaF affects the expression of IGF-IR in mouse osteoblasts, contributing to the proliferation and apoptosis induced by fluoride.
引用
收藏
页码:914 / 923
页数:10
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