Honeybees Apis mellifera can detect the scent of Mycobacterium tuberculosis

被引：35

作者：

Suckling, David M. ^{[1
]}

Sagar, Rachael L. ^{[1
]}

机构：

[1] New Zealand Inst Plant & Food Res Ltd, Christchurch 8140, New Zealand

来源：

TUBERCULOSIS | 2011年 / 91卷 / 04期

关键词：

Mycobacterium tuberculosis; Mycobacterium bovis; Methyl p-anisate; Methyl phenylacetate;

D O I：

10.1016/j.tube.2011.04.008

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The proboscis extension reflex in honeybees was evaluated for detection of tuberculosis. Restrained bees were tested with methyl phenylacetate, methyl p-anisate, and methyl nicotinate, previously identified from Mycobacterium tuberculosis cultures, to determine honeybee capacity for signature volatile detection. Methyl p-anisate and methyl phenylacetate were detectable over eight orders of magnitude, and honeybees showed proboscis extension response down to 0.1 pg loading of methyl p-anisate on filter paper. Potential exists for trained honeybees in non-invasive diagnostic tests for TB. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：327 / 328

页数：2

共 9 条

[1] [Anonymous], 2010, TUB FACT SHEET 104
[2] El-Sayed A.M., 2011, The pherobase: Database of insect pheromones and semiochemicals
[3] Prospects for clinical application of electronic-nose technology to early detection of Mycobacterium tuberculosis in culture and sputum
Fend, Reinhard
Kolk, Arend H. J.
Bessant, Conrad
Buijtels, Patricia
Klatser, Paul R.
Woodman, Anthony C.
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (06) : 2039 - 2045
[4] Menzel R., 1983, P206
[5] Facing the crisis: Improving the diagnosis of tuberculosis in the HIV era
Perkins, Mark D.
Cunningham, Jane
[J]. JOURNAL OF INFECTIOUS DISEASES, 2007, 196 : S15 - S27
[6] Asymmetrical generalisation between pheromonal and floral odours in appetitive olfactory conditioning of the honey bee (Apis mellifera L.)
Sandoz J.
Pham-Delègue M.
Renou M.
Wadhams L.
[J]. Journal of Comparative Physiology A, 2001, 187 (7): : 559 - 568
[7] The scent of Mycobacterium tuberculosis
Syhre, Mona
Chambers, Stephen T.
[J]. TUBERCULOSIS, 2008, 88 (04) : 317 - 323
[8] The scent of Mycobacterium tuberculosis - Part II breath
Syhre, Mona
Manning, Laurens
Phuanukoonnon, Suparat
Harino, Paul
Chambers, Stephen T.
[J]. TUBERCULOSIS, 2009, 89 (04) : 263 - 266
[9] von Frisch K., 1967, The Dance Language and Orientation of Bees, V2nd

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