SLA-DRB1 and-DQB1 genotyping by the PCR-SSOP-Luminex method

被引：10

作者：

Ando, A. ^{[1
]}

Shigenari, A. ^{[1
]}

Ota, M. ^{[2
]}

Sada, M. ^{[3
]}

Kawata, H. ^{[4
]}

Azuma, F. ^{[5
]}

Kojima-Shibata, C. ^{[6
]}

Nakajoh, M. ^{[6
]}

Suzuki, K. ^{[7
]}

Uenishi, H. ^{[8
]}

Kulski, J. K. ^{[1
,9
]}

Inoko, H. ^{[1
]}

机构：

[1] Tokai Univ, Sch Med, Div Basic Med Sci & Mol Med, Dept Mol Life Sci, Kanagawa 2591193, Japan

[2] Shinshu Univ, Sch Med, Dept Legal Med, Nagano, Japan

[3] Natl Cardiovasc Ctr, Dept Reprod Med, Osaka, Japan

[4] Tokai Univ, Sch Med, Teaching & Res Support Ctr, Kanagawa 2591193, Japan

[5] Med & Biol Labs Co Ltd, Ina Lab, Nagano, Japan

[6] Miyagi Livestock Expt Stn, Osaki, Miyagi, Japan

[7] Tohoku Univ, Grad Sch Agr Sci, Sendai, Miyagi 980, Japan

[8] Natl Inst Agrobiol Sci, Div Anim Sci, Tsukuba, Ibaraki, Japan

[9] Univ Western Australia, Ctr Forens Sci, Nedlands, WA 6009, Australia

来源：

TISSUE ANTIGENS | 2011年 / 78卷 / 01期

关键词：

genotyping; Luminex method; major histocompatibility complex; polymerase chain reaction-sequence-specific oligonucleotide probe; swine; swine leukocyte antigen; LEUKOCYTE ANTIGEN SLA; HLA-B; SWINE; IDENTIFICATION; SYSTEM; GENES; POLYMORPHISM; DISEASE; DNA; NOMENCLATURE;

D O I：

10.1111/j.1399-0039.2011.01669.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A simple and novel genotyping method was developed to detect alleles at the swine leukocyte antigen (SLA)-DRB1 and -DQB1 class II loci by using polymerase chain reaction (PCR)-fluorescently labeled sequence-specific oligonucleotide probes (SSOPs) and Luminex 100 xMAP detection. The PCR-SSOP-Luminex method exhibited accuracy of 95% for both SLA-DRB1 and -DQB1 in 6 homozygous and 16 heterozygous pig samples as confirmed by sequencing the PCR products of the same samples. In addition, 12 low-resolution SLA class II haplotypes consisting of 7 and 9 DRB1 and DQB1 alleles were identified, respectively, in one population of 283 Landrace pigs. This genotyping method facilitates the rapid and accurate identification of two- or four-digit alleles at the SLA-DRB1 and -DQB1 loci.

引用

页码：49 / 55

页数：7

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