Recombinant hemagglutinin proteins formulated in a novel PELC/CpG adjuvant for H7N9 subunit vaccine development

被引：5

作者：

Chen, Ting-Hsuan ^{[1
]}

Liu, Ying-Yu ^{[1
]}

Jan, Jia-Tsrong ^{[2
]}

Huang, Ming-Hsi ^{[3
]}

Spearman, Maureen ^{[4
]}

Butler, Michael ^{[4
]}

Wu, Suh-Chin ^{[1
,5
]}

机构：

[1] Natl Tsing Hua Univ, Inst Biotechnol, Hsinchu 30013, Taiwan

[2] Acad Sinica, Genom Res Ctr, Taipei 11529, Taiwan

[3] Natl Hlth Res Inst, Natl Inst Infect Dis & Vaccinol, Zhunan, Taiwan

[4] Univ Manitoba, Dept Microbiol, Winnipeg, MB, Canada

[5] Natl Tsing Hua Univ, Dept Med Sci, Hsinchu 30013, Taiwan

来源：

ANTIVIRAL RESEARCH | 2017年 / 146卷

关键词：

PELC/CpG; Adjuvant; H7N9; Subunit vaccine; INFLUENZA A(H7N9) VIRUS; RECEPTOR-BINDING; HUMAN INFECTIONS; IMMUNE-RESPONSE; MF59; ADJUVANT; A VIRUS; IMMUNOGENICITY; PROTECTION; E627K; MICE;

D O I：

10.1016/j.antiviral.2017.09.014

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Humans infected with H7N9 avian influenza viruses can result in severe pneumonia and acute respiratory syndrome with an approximately 40% mortality rate, and there is an urgent need to develop an effective vaccine to reduce its pandemic potential. In this study, we used a novel PELC/CpG adjuvant for recombinant H7HA (rH7HA) subunit vaccine development. After immunizing BALB/c mice intramuscularly, rH7HA proteins formulated in this adjuvant instead of an alum adjuvant elicited higher IgG, hemagglutination-inhibition, and virus neutralizing antibodies in sera; induced higher numbers of H7HA-specific IFN-y-secreting T cells and antibody secreting cells in spleen; and provided improved protection against live virus challenges. Our results indicate that rH7HA proteins formulated in PELC/CpG adjuvant can induce potent anti-H7N9 immunity that may provide useful information for H7N9 subunit vaccine development.

引用

页码：213 / 220

页数：8

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