Recombinant hemagglutinin proteins formulated in a novel PELC/CpG adjuvant for H7N9 subunit vaccine development

被引：5
作者：
Chen, Ting-Hsuan [1 ]
Liu, Ying-Yu [1 ]
Jan, Jia-Tsrong [2 ]
Huang, Ming-Hsi [3 ]
Spearman, Maureen [4 ]
Butler, Michael [4 ]
Wu, Suh-Chin [1 ,5 ]
机构：
[1] Natl Tsing Hua Univ, Inst Biotechnol, Hsinchu 30013, Taiwan
[2] Acad Sinica, Genom Res Ctr, Taipei 11529, Taiwan
[3] Natl Hlth Res Inst, Natl Inst Infect Dis & Vaccinol, Zhunan, Taiwan
[4] Univ Manitoba, Dept Microbiol, Winnipeg, MB, Canada
[5] Natl Tsing Hua Univ, Dept Med Sci, Hsinchu 30013, Taiwan
来源：
ANTIVIRAL RESEARCH | 2017年 / 146卷
关键词：
PELC/CpG; Adjuvant; H7N9; Subunit vaccine; INFLUENZA A(H7N9) VIRUS; RECEPTOR-BINDING; HUMAN INFECTIONS; IMMUNE-RESPONSE; MF59; ADJUVANT; A VIRUS; IMMUNOGENICITY; PROTECTION; E627K; MICE;
D O I：
10.1016/j.antiviral.2017.09.014
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Humans infected with H7N9 avian influenza viruses can result in severe pneumonia and acute respiratory syndrome with an approximately 40% mortality rate, and there is an urgent need to develop an effective vaccine to reduce its pandemic potential. In this study, we used a novel PELC/CpG adjuvant for recombinant H7HA (rH7HA) subunit vaccine development. After immunizing BALB/c mice intramuscularly, rH7HA proteins formulated in this adjuvant instead of an alum adjuvant elicited higher IgG, hemagglutination-inhibition, and virus neutralizing antibodies in sera; induced higher numbers of H7HA-specific IFN-y-secreting T cells and antibody secreting cells in spleen; and provided improved protection against live virus challenges. Our results indicate that rH7HA proteins formulated in PELC/CpG adjuvant can induce potent anti-H7N9 immunity that may provide useful information for H7N9 subunit vaccine development.
引用
收藏
页码：213 / 220
页数：8
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