Determination of deoxyribonucleic acids by a resonance light scattering technique and its application

被引：13

作者：

Jie, NQ ^{[1
]}

Jia, GF ^{[1
]}

Hou, SC ^{[1
]}

Xiong, YM ^{[1
]}

Dong, YH ^{[1
]}

机构：

[1] China Agr Univ, Coll Appl Chem, Beijing 100094, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2003年 / 59卷 / 14期

关键词：

acetamiprid; resonance light scattering method; DNA;

D O I：

10.1016/S1386-1425(03)00155-0

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

For the first time, acetamiprid has been used to determine nucleic acid (DNA) using the resonance light scattering (RLS). The RLS of acetamiprid was greatly enhanced by DNA in the range of pH 1.6-1.8. A RLS peak at 313 nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0-11.0 mug ml(-1) with the detection limit of 20 ng ml(-1). The nucleic acids in synthetic sample and in rice seedling extraction were determined satisfactorily. The interaction mechanism of acetamiprid and DNA is discussed. Mechanism studies show that the enhanced RLS is due to the aggregation of acetamiprid in the presence of DNA. (C) 2003 Published by Elsevier B.V.

引用

页码：3295 / 3301

页数：7

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