Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum

被引：7

作者：

Bhatia, S. ^{[1
]}

Sood, Richa ^{[1
]}

Bhatia, A. K. ^{[2
]}

Pattnaik, B. ^{[1
]}

Pradhan, H. K. ^{[1
]}

机构：

[1] Indian Vet Res Inst, High Secur Anim Dis Lab, Bhopal 462021, Madhya Pradesh, India

[2] UP Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vis, Coll Vet Sci & Anim Husb, Dept Microbiol & Immunol, Mathura 281001, UP, India

来源：

JOURNAL OF VIROLOGICAL METHODS | 2008年 / 148卷 / 1-2期

关键词：

BIV; capsid; competitive ELISA; gag; monoclonal antibody;

D O I：

10.1016/j.jviromet.2007.11.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to develop a more specific and sensitive competitive inhibition ELISA (CI-ELISA) than the currently used indirect ELISA for detection of antibodies to bovine immunodeficiency virus (BIV) in cattle and buffaloes. Murine monoclonal antibodies (MAbs) were generated against a recombinant capsid (CA) protein of bovine immunodeficiency virus. Of the 13 anti-CA MAbs developed, MAb-9G10 was selected for CI-ELISA based on the maximum inhibition (98%) obtained with reference BIV antibody positive serum. Based on the distribution of percent inhibition of known negative sera (n = 50), a cut-off value was set at 40% inhibition. The MAb-based CI-ELISA showed much higher agreement (concordance: 95.4%) than the indirect ELISA (concordance: 77.8%) with Western blot. Out of 672 sera of cattle and buffaloes tested by CI-ELISA from four states of India, 22% (113/516) of cattle and 19% (30/156) of buffalo were sero-positive for BIV with an overall seroprevalence of 21% (143/672) in India. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：218 / 225

页数：8

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