Ultrasonic-assisted extraction of polysaccharides from coix seeds: Optimization, purification, and in vitro digestibility

被引：128

作者：

Hu, Xintian ^{[1
]}

Xu, Feiran ^{[2
]}

Li, Jinglei ^{[2
]}

Li, Jun ^{[1
]}

Mo, Cheng ^{[1
]}

Zhao, Meng ^{[1
]}

Wang, Lifeng ^{[1
]}

机构：

[1] Nanjing Univ Finance & Econ, Coll Food Sci & Engn, Collaborat Innovat Ctr Modern Grain Circulat & Sa, Nanjing 210023, Jiangsu, Peoples R China

[2] Hefei Univ Technol, Sch Food & Biol Engn, Engn Res Ctr Bioproc, Minist Educ, Hefei 230009, Peoples R China

来源：

FOOD CHEMISTRY | 2022年 / 374卷

基金：

中国国家自然科学基金;

关键词：

Coix seeds polysaccharides; Ultrasonic-assisted extraction; Response surface methodology; Structural properties; In vitro digestibility; PLANTAGO-ASIATICA L; ANTIOXIDANT ACTIVITY; STRUCTURAL-CHARACTERIZATION; ADLAY; FERMENTATION;

D O I：

10.1016/j.foodchem.2021.131636

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

To optimize the extraction of polysaccharides from coix seeds (CSP), an auxiliary method of ultrasound was developed by response surface methodology (RSM). The maximum extraction yield (8.340%) was obtained under 480 W power, 16 min ultrasound extraction (UE) time and 21.00 mL/g water to raw material ratio. Compared to hot water extraction (HE), UE-treated CSP led to a higher extraction efficiency and decreased average CSP molecular weight. FT-IR indicated that CSP extracted by UE and HE were neutral polysaccharides, and linkages between sugar units were mainly in the alpha-conformation. Furthermore, NMR spectra indicated that UE-treated CSP was a neutral polysaccharide with (1 -> 6)-linked alpha-D-glucopyranose in the main chain. Two polysaccharide components (CSP-A and CSP-B) were purified by anion exchange chromatography, therein, CSP-A was more resistant to the digestion in stomach and intestine. These results suggest that CSP-A has the potential to be a functional agent utilized by gut microbes.

引用

页数：10

共 40 条

[1] Obtaining extracts rich in antioxidant polysaccharides from the edible mushroom Pleurotus ostreatus using binary system with hot water and supercritical CO2 [J].