Analysis of 16S rDNA sequences from citrus huanglongbing bacteria reveal a different "Ca. Liberibacter" strain associated with citrus disease in Sao Paulo

被引：50

作者：

Coletta, HD ^{[1
]}

Takita, MA ^{[1
]}

Targon, MLPN ^{[1
]}

Machado, MA ^{[1
]}

机构：

[1] Inst Agron, Ctr APTA Citros Sylvio Moreira, BR-13490970 Cordeiropolis, SP, Brazil

来源：

PLANT DISEASE | 2005年 / 89卷 / 08期

关键词：

PCR; sweet orange;

D O I：

10.1094/PD-89-0848

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Citrus huanglongbing (HLB, ex greening) is one of the most serious and destructive citrus diseases in the world. It is caused by a phloem-limited and nonculturable bacterium, "Candidatus Liberibacter". The disease occurs in some Asian and African countries and recently has been reported in the state of Sao Paulo, Brazil. Analysis of the 16S ribosomal (r)DNA of the HLB bacteria from orchards in Sao Paulo revealed the presence of two distinct strains of "Ca. Liberibacter". One of them, named LSg1 (Liberibacter sequence group 1), was 100% identical to strains from Japan (GenBank accessions AB038369 and AB008366), the Asian forms of the bacteria. The other, LSg2, is genetically distant from the Asian (96.1 to 96.3% identity) and African (95.8 to 96.1% identity) strains. Comparison of the 16S rDNA sequences from the LSg2 and the Asian strain revealed the presence of INDELs and point mutations. Specific primers designed for this Brazilian Liberibacter strain revealed that it is more widely distributed throughout the Sao Paulo orchards compared with the LSg1 strain. The HLB symptoms caused by both strains are almost identical and, interestingly, both strains were found in the same sample, revealing mixed infection in a citrus plant.

引用

页码：848 / 852

页数：5

共 22 条

[1]

Bove J. M., 1974, Proceedings of the Sixth Conference of the International Organization of Citrus Virologists., P12

[2] First report of the causal agent of huanglongbing ("Candidatus Liberibacter asiaticus") in Brazil. [J].

Coletta, HD ;

Targon, MLPN ;

Takita, MA ;

De Negri, JD ;

Pompeu, J ;

Machado, MA ;

Muller, GW .

PLANT DISEASE, 2004, 88 (12) :1382-1382

[3]

GALLO D., 1960, REV AGRIC [PIRACICABA], V35, P41

[4] Genomic characterization of a liberibacter present in an ornamental rutaceous tree, Calodendrum capense, in the Western Cape province of South Africa.: Proposal of 'Candidatus Liberibacter africanus subsp capensis' [J].

Garnier, M ;

Jagoueix-Eveillard, S ;

Cronje, PR ;

Le Roux, HF ;

Bové, JM .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :2119-2125

[5]

GRAVENA S, 1996, P 13 C INT ORG CITR, P414

[6]

Halbert S.E., 2003, P INT SOC CITRICULTU, P1016

[7]

Halbert SE, 2004, FLA ENTOMOL, V87, P330, DOI 10.1653/0015-4040(2004)087[0330:ACPSPA]2.0.CO

[8]

[9]

Harakava R., 2000, Proceedings of the 14th Conference of the International Organization of Citrus Virologists, Campinas, Sao Paulo State, Brazil, 13-18 September 1998, P195

[10] Detection and identification of the two Candidatus Liberobacter species associated with citrus huanglongbing by PCR amplification of ribosomal protein genes of the β operon [J].

Hocquellet, A ;

Toorawa, P ;

Bové, JM ;

Garnier, M .

MOLECULAR AND CELLULAR PROBES, 1999, 13 (05) :373-379

← 1 2 3 →