CYP1A1 and MT1K are congener specific biomarker genes for liver diseases induced by PCBs

被引：13

作者：

Dutta, Sisir K. ^{[1
]}

Ghosh, Somiranjan ^{[1
]}

De, Supriyo ^{[1
]}

Hoffman, Eric P. ^{[2
]}

机构：

[1] Howard Univ, Dept Biol, Mol Genet Lab, Washington, DC 20059 USA

[2] Childrens Natl Med Hosp, Ctr Genet Med, Washington, DC 20010 USA

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2008年 / 25卷 / 02期

关键词：

PCB-77; PCB-153; biomarker genes; CYP1A1; MT1K;

D O I：

10.1016/j.etap.2007.10.018

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

We have identified two most significant biomarker genes, CYP1A1 (69.81 up-regulation) and MT1K (14.66 up-regulation), showing highest overexpression at p-value <0.005. These were selected out of several hundred genes induced in vitro, using PCB exposed human liver (HepG2) cells. Over expression of the CYP1A1 (cytochrome P450) gene was specific to PCB-77 and MT1K (Metallothionein) to PCB-153. Affymetrix oligonucleotide microarrays (mRNA) were used to screen the entire genome of human liver cells in a time-dependent exposure and were further validated by quantitative real-time RT-PCR. (C) 2007 Published by Elsevier B.V.

引用

页码：218 / 221

页数：4

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