Molecular characterization of two cultivars of Egyptian clover (Trifolium alexandrinum L.)

被引:0
作者
Zayed, E. M. [1 ]
Soliman, Magda I. [2 ]
Ramadan, G. A. [1 ]
Tarrad, M. M. [1 ]
机构
[1] Agr Res Ctr, Field Crops Res Inst, Forage Crops Res Dept, Giza 12619, Egypt
[2] Mansoura Univ, Fac Sci, Dept Bot, Mansoura, Egypt
关键词
AFLP; Egyptian clover; Fodder; ISSR; Molecular markers; RAPD; Trifolium; GENETIC DIVERSITY; PRATENSE L; MARKERS; POPULATIONS;
D O I
暂无
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Two Egyptian clover cultivars namely 'Fahl' and 'Helaly' were analyzed based on seed soluble protein pattern as well as RAPD, ISSR and AFLP generated DNA profiles. For AFLP analysis, DNA of Egyptian clover cultivars were digested with EcoRI + Msel restriction enzymes. Among the molecular markers used ISSR showed highest level of molecular variance (24.5%). In terms of allele frequency (p), level of difference between two cultivars was variable. Results also indicated that relationship study between two cultivars can be better performed using Cubic, Quadratic model.
引用
收藏
页码:140 / 143
页数:4
相关论文
共 18 条
[1]   Genetic transformation of wheat mediated by Agrobacterium tumefaciens [J].
Cheng, M ;
Fry, JE ;
Pang, SZ ;
Zhou, HP ;
Hironaka, CM ;
Duncan, DR ;
Conner, TW ;
Wan, YC .
PLANT PHYSIOLOGY, 1997, 115 (03) :971-980
[2]  
DAN M, 1997, MOL BREEDING, V3, P127
[3]  
Dellaporta S, 1983, Plant Mol BiolRep, V1, P19, DOI DOI 10.1007/BF02712670
[4]   The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin [J].
Fernández, ME ;
Figueiras, AM ;
Benito, C .
THEORETICAL AND APPLIED GENETICS, 2002, 104 (05) :845-851
[5]  
GARDINIER E, 1988, LENINGRAD, V16, P65
[6]   Relating morphologic and RAPD marker variation to collection site environment in wild populations of red clover (Trifolium pratense L.) [J].
Greene, SL ;
Gritsenko, M ;
Vandemark, G .
GENETIC RESOURCES AND CROP EVOLUTION, 2004, 51 (06) :643-653
[7]   Variability of storage proteins and esterase isozymes in Vicia sativa subspecies [J].
Haider, AS ;
El-Shanshoury, AR .
BIOLOGIA PLANTARUM, 2000, 43 (02) :205-209
[8]  
LAWLER FC, 2003, PCR METH APPL, V2, P275
[9]   Mixed grazing and climatic determinants of white clover (Trifolium repens L.) content in a permanent pasture [J].
Nolan, T ;
Connolly, J ;
Wachendorf, M .
ANNALS OF BOTANY, 2001, 88 :713-724
[10]  
Rafalski J A, 1996, ANAL NONMAMMALIAN GE