Microbispora soli sp. nov., isolated from soil of a hot spring

A novel actinomycete, strain RTBAU4-9(T), which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area. This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H-4), MK-9(H-2) and MK-9(H-0) as the characteristic menaquinones, and iso-C-16:0 and anteiso-C-17:0 as the main fatty acids. The G+C content of the genomic DNA was 70.2 mol%. The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020(T) (98.3 % 16S rRNA gene sequence similarity). In addition, the low percentage of DNA-DNA relatedness (<28.1 +/- 1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9(T) should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp. nov. is proposed. The type strain is RTBAU4-9(T) (=TBRC 7648(T) =NBRC 113147(T)).