Extraction of antioxidant pectic-polysaccharide from mangosteen (Garcinia mangostana) rind Optimization using response surface methodology

Box-Behnken design (BBD) was employed to optimize the incubator temperature (X(1) 50-80 C) extraction time (X(2) 2-4 h) and pH (X(3) 2-4) to obtain a high antioxidant pectic-polysaccharide yield with high worm acid content and antioxidant activity from mangosteen rind Analysis of variance showed that the contribution of quadratic model was significant for the extraction yield and antioxidant activity whereas linear model was significant for pectin content Optimization study using response surface methodology was performed and 3D response surfaces were plotted from the mathematical model Two optimal conditions were given condition (1)X(1) = 800 C X(2) = 3 93 h X(3) = 245 and condition (2)X(1) = 67 7 C X(2) = 367 h X3 = 200 These optimum conditions yielded pectic-polysaccharide of similar to 12 0-12 4% uronic acid content of similar to 20 2-21 1 mg/g and %DPPHsc/g extract of 225-252 respectively Close agreement between experimental and predicted values was found This could therefore be applied in extraction of nnangosteen-derived functional pectic-polysaccharide in industry (C) 2010 Elsevier Ltd All rights reserved