Monitoring drug nanocarriers in human blood by near-infrared fluorescence correlation spectroscopy

被引：62

作者：

Negwer, Inka ^{[1
,2
]}

Best, Andreas ^{[1
]}

Schinnerer, Meike ^{[3
,4
]}

Schaefer, Olga ^{[4
]}

Capeloa, Leon ^{[4
]}

Wagner, Manfred ^{[1
]}

Schmidt, Manfred ^{[3
]}

Mailaender, Volker ^{[1
,5
]}

Helm, Mark ^{[2
]}

Barz, Matthias ^{[4
]}

Butt, Hans-Juergen ^{[1
,6
]}

Koynov, Kaloian ^{[1
]}

机构：

[1] Max Planck Inst Polymer Res, Ackermannweg 10, D-55128 Mainz, Germany

[2] Johannes Gutenberg Univ Mainz, Inst Pharm & Biochem, Pharmaceut Chem, Staudinger Weg 5, D-55128 Mainz, Germany

[3] Johannes Gutenberg Univ Mainz, Inst Phys Chem, Jakob Welder Weg 11, D-55128 Mainz, Germany

[4] Johannes Gutenberg Univ Mainz, Inst Organ Chem, Duesbergweg 10-14, D-55128 Mainz, Germany

[5] Johannes Gutenberg Univ Mainz, Univ Med Ctr, Dept Dermatol, Langenbeckstr 1, D-55131 Mainz, Germany

[6] Tokyo Inst Technol, Earth Life Sci Inst, Meguro Ku, Tokyo 1528551, Japan

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

关键词：

PHYSICOCHEMICAL CHARACTERIZATION; PROTEIN ADSORPTION; NANOPARTICLES; BINDING; CONJUGATION; CHALLENGES; PRINCIPLES; PARTICLES; STABILITY; POLYMERS;

D O I：

10.1038/s41467-018-07755-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nanocarrier-based drug delivery is a promising therapeutic approach that offers unique possibilities for the treatment of various diseases. However, inside the blood stream, nanocarriers' properties may change significantly due to interactions with proteins, aggregation, decomposition or premature loss of cargo. Thus, a method for precise, in situ characterization of drug nanocarriers in blood is needed. Here we show how the fluorescence correlation spectroscopy that is a well-established method for measuring the size, loading efficiency and stability of drug nanocarriers in aqueous solutions can be used to directly characterize drug nanocarriers in flowing blood. As the blood is not transparent for visible light and densely crowded with cells, we label the nanocarriers or their cargo with near-infrared fluorescent dyes and fit the experimental autocorrelation functions with an analytical model accounting for the presence of blood cells. The developed methodology contributes towards quantitative understanding of the in vivo behavior of nanocarrier-based therapeutics.

引用

页数：9