Improving the detection of IgM antibodies against glycolipids complexes of GM1 and Galactocerebroside in Multifocal Motor Neuropathy using glycoarray and ELISA assays

被引：16

作者：

Delmont, Emilien ^{[1
,2
]}

Halstead, Susan ^{[1
]}

Galban-Horcajo, Francesc ^{[1
]}

Yao, Denggao ^{[1
]}

Desnuelle, Claude ^{[2
]}

Willison, Hugh ^{[1
]}

机构：

[1] Univ Glasgow, Coll Med Vet & Life Sci, Inst Infect Immun & Inflammat, Glasgow G12 8QQ, Lanark, Scotland

[2] Referral Ctr ALS & Neuromuscular Dis, F-06200 Nice, France

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2015年 / 278卷

关键词：

Multifocal motor neuropathy; Anti-ganglioside antibodies; Gangliosides complexes; ELISA; DIAGNOSTIC-VALUE; VALIDATION;

D O I：

10.1016/j.jneuroim.2014.11.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Antibodies against complexes of GM1:GalC are detected in multifocal motor neuropathy. Previous studies used different techniques, explaining disparities in the results. Antibodies against GM1 and GM1:GalC with different proportions of GalC were measured with both glycoarray and ELISA in 20 multifocal motor neuropathies, and 45 controls. The 1:5 ratio and the 1:1 ratio of GM1:GalC (weight ratio) were respectively the most effective for glycoarray and for ELISA. Testing for anti-GM 1:GalC antibodies increased the sensitivity from 40% with anti-GMI antibodies to 65% with array and 60% with ELISA without loss in specificity (above 91%). Anti-GMI: GalC antibodies are effective biological tools to diagnose multifocal motor neuropathy. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：159 / 161

页数：3

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