Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ

被引：80

作者：

Pagliuso, Alessandro ^{[1
,2
,5
]}

Valente, Carmen ^{[1
]}

Giordano, Lucia Laura ^{[1
]}

Filograna, Angela ^{[1
]}

Li, Guiling ^{[1
,6
]}

Circolo, Diego ^{[1
]}

Turacchio, Gabriele ^{[1
]}

Marzullo, Vincenzo Manuel

Mandrich, Luigi ^{[1
]}

Zhukovsky, Mikhail A. ^{[1
]}

Formiggini, Fabio ^{[4
]}

Polishchuk, Roman S. ^{[2
]}

Corda, Daniela ^{[1
]}

Luini, Alberto ^{[1
,3
]}

机构：

[1] CNR, Inst Prot Biochem, Via Pietro Castellino 111, I-80131 Naples, Italy

[2] Telethon Inst Genet & Med TIGEM, Via Campi Flegrei 34, I-80078 Pozzuoli, Italy

[3] IRCCS SDN Ist Ric Diagnost & Nucl, Via Emanuele Gianturco 113, I-80143 Naples, Italy

[4] Italian Inst Technol, Ctr Adv Biomat Hlth Care CRIB, Largo Barsanti & Matteucci 53, I-80125 Naples, Italy

[5] Inst Pasteur, Unite Interact Bacteries Cellules, Dept Biol Cellulaire & Infect, 25-28 Rue Docteur Roux, F-75015 Paris, France

[6] Wenzhou Med Univ, Inst Genom Med, Wenzhou 325000, Zhejiang, Peoples R China

来源：

NATURE COMMUNICATIONS | 2016年 / 7卷

关键词：

POLARIZED MDCK CELLS; MOLECULAR-MECHANISM; PHOSPHATIDIC-ACID; ANCHORED PROTEINS; TRANSPORT; CTBP1/BARS; CTBP/BARS; DYNAMIN; DOMAIN; COMPLEX;

D O I：

10.1038/ncomms12148

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Membrane fission is an essential cellular process by which continuous membranes split into separate parts. We have previously identified CtBP1-S/BARS (BARS) as a key component of a protein complex that is required for fission of several endomembranes, including basolateral post-Golgi transport carriers. Assembly of this complex occurs at the Golgi apparatus, where BARS binds to the phosphoinositide kinase PI4KIIIb through a 14-3-3 gamma dimer, as well as to ARF and the PKD and PAK kinases. We now report that, when incorporated into this complex, BARS binds to and activates a trans-Golgi lysophosphatidic acid (LPA) acyltransferase type delta (LPAAT delta) that converts LPA into phosphatidic acid (PA); and that this reaction is essential for fission of the carriers. LPA and PA have unique biophysical properties, and their interconversion might facilitate the fission process either directly or indirectly (via recruitment of proteins that bind to PA, including BARS itself).

引用

页数：15

共 70 条

[1] N-terminal hydrophobic residues of the G-protein ADP-ribosylation factor-1 insert into membrane phospholipids upon GDP to GTP exchange [J].

Antonny, B ;

BeraudDufour, S ;

Chardin, P ;

Chabre, M .

BIOCHEMISTRY, 1997, 36 (15) :4675-4684

[2] RECONSTITUTION OF THE TRANSPORT OF PROTEIN BETWEEN SUCCESSIVE COMPARTMENTS OF THE GOLGI MEASURED BY THE COUPLED INCORPORATION OF N-ACETYLGLUCOSAMINE [J].

BALCH, WE ;

DUNPHY, WG ;

BRAELL, WA ;

ROTHMAN, JE .

CELL, 1984, 39 (02) :405-416

[3] Role of diacylglycerol in PKD recruitment to the TGN and protein transport to the plasma membrane [J].

Baron, CL ;

Malhotra, V .

SCIENCE, 2002, 295 (5553) :325-328

[4] CtBP3/BARS drives membrane fission in dynamin-independent transport pathways [J].

Bonazzi, M ;

Spanò, S ;

Turacchio, G ;

Cericola, C ;

Valente, C ;

Colanzi, A ;

Kweon, HS ;

Hsu, VW ;

Polishchuck, EV ;

Polishchuck, RS ;

Sallese, M ;

Pulvirenti, T ;

Corda, D ;

Luini, A .

NATURE CELL BIOLOGY, 2005, 7 (06) :570-U11

[5] The tale of tail-anchored proteins: coming from the cytosol and looking for a membrane [J].

Borgese, N ;

Colombo, S ;

Pedrazzini, E .

JOURNAL OF CELL BIOLOGY, 2003, 161 (06) :1013-1019

[6] Membrane Fission Is Promoted by Insertion of Amphipathic Helices and Is Restricted by Crescent BAR Domains [J].

Boucrot, Emmanuel ;

Pick, Adi ;

Camdere, Gamze ;

Liska, Nicole ;

Evergren, Emma ;

McMahon, Harvey T. ;

Kozlov, Michael M. .

CELL, 2012, 149 (01) :124-136

[7] Acylglycerophosphate acyltransferase 4 (AGPAT4) is a mitochondrial lysophosphatidic acid acyltransferase that regulates brain phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol levels [J].

Bradley, Ryan M. ;

Marvyn, Phillip M. ;

Henao, Juan J. Aristizabal ;

Mardian, Emily B. ;

George, Steve ;

Aucoin, Marc G. ;

Stark, Ken D. ;

Duncan, Robin E. .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2015, 1851 (12) :1566-1576

[8] Membrane Fission: The Biogenesis of Transport Carriers [J].

Campelo, Felix ;

Malhotra, Vivek .

ANNUAL REVIEW OF BIOCHEMISTRY, VOL 81, 2012, 81 :407-427

[9] Control Systems of Membrane Transport at the Interface between the Endoplasmic Reticulum and the Golgi [J].

Cancino, Jorge ;

Capalbo, Anita ;

Di Campli, Antonella ;

Giannotta, Monica ;

Rizzo, Riccardo ;

Jung, Juan E. ;

Di Martino, Rosaria ;

Persico, Maria ;

Heinklein, Petra ;

Sallese, Michele ;

Luini, Alberto .

DEVELOPMENTAL CELL, 2014, 30 (03) :280-294

[10] Mitotic Golgi partitioning is driven by the membrane-fissioning protein CtBP3/BARS [J].

Carcedo, CH ;

Bonazzi, M ;

Spanò, S ;

Turacchio, G ;

Colanzi, A ;

Luini, A ;

Corda, D .

SCIENCE, 2004, 305 (5680) :93-96

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