Review of methods for determination of total protein and peptide concentration in biological samples

被引：45

作者：

Sapan, Christine V. ^{[1
]}

Lundblad, Roger L. ^{[1
]}

机构：

[1] Asana Med Inc, Miami, FL USA

来源：

PROTEOMICS CLINICAL APPLICATIONS | 2015年 / 9卷 / 3-4期

关键词：

Protein analysis; Sample prefractionation; Total peptide concentration; Total protein concentration; COOMASSIE BRILLIANT BLUE; SODIUM DODECYL-SULFATE; BICINCHONINIC ACID; DYE-BINDING; MICROGRAM QUANTITIES; QUANTITATION ASSAYS; BIURET REACTION; LOWRY METHOD; AMIDO BLACK; SERUM;

D O I：

10.1002/prca.201400088

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Clinical proteomics can be defined as the use of proteomic technologies to identify and measure biomarkers in fluids and tissues. The current work is intended to review various methods used for the determination of the total concentration of protein or peptide in fluids and tissues and the application of such methods to clinical proteomics. Specifically, this article considers the approaches to the measurement of total protein concentration, not the measurement of the concentration of a specific protein or group of proteins in a larger mixture of proteins. The necessity of understanding various concepts such as fit-for-use, quality-by-design, and other regulatory elements is discussed, as is the significance of using suitable standards for the protein quality of various samples.

引用

页码：268 / 276

页数：9

共 90 条

[1] Mechanism of Coomassie Brilliant Blue G-250 binding to cetyltrimethylammonium bromide: An interference with the Bradford assay [J].