Effects of a high-fiber diet on hepatocyte-apoptosis and liver regeneration after partial hepatectomy in rats with fatty liver

被引:12
|
作者
Lai, Hong-Shiee
Lin, Wen-His
Chen, Pey-Rong
Wu, Hsiu-Chuan
Lee, Po-Huang
Chen, Wei-Jao
机构
[1] Natl Taiwan Univ Hosp, Dept Surg, Taipei, Taiwan
[2] Natl Taiwan Univ Hosp, Dept Dietet, Taipei, Taiwan
[3] Natl Taiwan Univ, Coll Med, Taipei 10764, Taiwan
关键词
D O I
10.1177/0148607105029006401
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Background: The morbidity and mortality that accompany fatty liver may occur as a result of increased apoptosis of hepatocytes and decreased liver regeneration. We determined the effects of a high-fiber diet on hepatocyte apoptosis and liver regeneration after partial hepatectomy in rats with fatty liver. Methods: Fatty liver was induced in male Wistar rats weighing around 200 g through feeding of a high-fat diet for 4 weeks. The rats were then randomly assigned to 3 groups that received a high-fat diet, a normal diet, or a high-fiber diet for another 4 weeks. Partial hepatectomy (around 70%) was performed, and rats were killed 6, 24, 48, or 72 hours after hepatectomy. We then measured (1) the ratio of remnant liver weight to body weight and assessed the histology of the remnant liver as indicators of fatty liver, (2) caspase-3 activity and in situ cell death detection of DNA fragmentation as indicators of apoptosis, and (3) 5-bromo-2-deoxyuridine (BrdU) activity and (4) ornithine decarboxylase (ODC) contents in remnant livers as markers of regeneration. Results: We found that (1) a high-fat diet for 4 weeks can markedly induce fatty liver, (2) apoptosis of hepatocytes is greater in fatty liver than in normal liver (98 +/- 19 us 36 +/- 7) at 6 hours after partial hepatectomy (p <.05), (3) the capacity of liver regeneration decreases significantly (BrdU index: 30 +/- 5 us 12 +/- 3, and ODC contents: 604 +/- 48 vs 390 +/- 42 mg/dL) in fatty liver 24 hours after partial hepatectomy (p <.05), (4) a normal diet can partially reverse the effects of fatty liver; (5) a high-fiber diet can significantly reverse the effects of fatty liver (p <.05). Conclusions: A high-fiber diet can reverse the negative effects of fatty liver on apoptosis and regenerative capacity after partial hepatectomy.
引用
收藏
页码:401 / 407
页数:7
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