FGF9 Suppresses Meiosis and Promotes Male Germ Cell Fate in Mice

被引：192

作者：

Bowles, Josephine ^{[1
,2
]}

Feng, Chun-Wei ^{[1
]}

Spiller, Cassy ^{[1
,2
]}

Davidson, Tara-Lynne ^{[1
,2
]}

Jackson, Andrew ^{[1
,2
]}

Koopman, Peter ^{[1
,2
]}

机构：

[1] Univ Queensland, Div Mol Genet & Dev, Brisbane, Qld 4072, Australia

[2] Univ Queensland, Inst Mol Biosci, ARC Ctr Excellence Biotechnol & Dev, Brisbane, Qld 4072, Australia

来源：

DEVELOPMENTAL CELL | 2010年 / 19卷 / 03期

基金：

英国医学研究理事会; 澳大利亚研究理事会;

关键词：

RETINOIC ACID; SEX DETERMINATION; PLURIPOTENT CELLS; CONSERVED ROLE; OPPOSING FGF; MOUSE GONADS; BODY AXIS; DIFFERENTIATION; EXPRESSION; FETAL;

D O I：

10.1016/j.devcel.2010.08.010

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Sex determination of mammalian germ cells occurs during fetal development and depends on signals from gonadal somatic cells. Previous studies have established that retinoic acid (RA) triggers ovarian germ cells to enter meiosis and thereby commit to oogenesis, whereas in the developing testis, the enzyme CYP26B1 degrades RA and germ cells are not induced to enter meiosis. Using in vitro and in vivo models, we demonstrate that fibroblast growth factor 9 (FGF9) produced in the fetal testis acts directly on germ cells to inhibit meiosis; in addition, FGF9 maintains expression of pluripotency-related genes and upregulates markers associated with male germ cell fate. We conclude that two independent and mutually antagonistic pathways involving RA and FGF9 act in concert to determine mammalian germ cell sexual fate commitment and support a model in which the mitosis/meiosis switch is robustly controlled by both positive and negative regulatory factors.

引用

页码：440 / 449

页数：10

共 46 条

[1]

Adams IR, 2002, DEVELOPMENT, V129, P1155

[2] In germ cells of mouse embryonic ovaries, the decision to enter meiosis precedes premeiotic DNA replication [J].

Baltus, Andrew E. ;

Menke, Douglas B. ;

Hu, Yueh-Chiang ;

Goodheart, Mary L. ;

Carpenter, Anne E. ;

de Rooij, Dirk G. ;

Page, David C. .

NATURE GENETICS, 2006, 38 (12) :1430-1434

[3] Opposing effects of retinoic acid and FGF9 on Nanos2 expression and meiotic entry of mouse germ cells [J].

Barrios, Florencia ;

Filipponi, Doria ;

Pellegrini, Manuela ;

Paronetto, Maria Paola ;

Di Siena, Sara ;

Geremia, Raffaele ;

Rossi, Pellegrino ;

De Felici, Massimo ;

Jannini, Emmanuele A. ;

Dolci, Susanna .

JOURNAL OF CELL SCIENCE, 2010, 123 (06) :871-880

[4] Sdmg1 is a conserved transmembrane protein associated with germ cell sex determination and germline-soma interactions in mice [J].

Best, Diana ;

Sahlender, Daniela A. ;

Walther, Norbert ;

Peden, Andrew A. ;

Adams, Ian R. .

DEVELOPMENT, 2008, 135 (08) :1415-1425

[5] Retinoid signaling determines germ cell fate in mice [J].

Bowles, J ;

Knight, D ;

Smith, C ;

Wilhelm, D ;

Richman, J ;

Mamiya, S ;

Yashiro, K ;

Chawengsaksophak, K ;

Wilson, MJ ;

Rossant, J ;

Hamada, H ;

Koopman, P .

SCIENCE, 2006, 312 (5773) :596-600

[6] Retinoic acid, meiosis and germ cell fate in mammals [J].

Bowles, Josephine ;

Koopman, Peter .

DEVELOPMENT, 2007, 134 (19) :3401-3411

[7] INDUCTION OF MEIOSIS IN FETAL MOUSE TESTIS INVITRO [J].

BYSKOV, AG ;

SAXEN, L .

DEVELOPMENTAL BIOLOGY, 1976, 52 (02) :193-200

[8] DOES RETE OVARII ACT AS A TRIGGER FOR ONSET OF MEIOSIS [J].

BYSKOV, AGS .

NATURE, 1974, 252 (5482) :396-397

[9]

Colvin JS, 2001, DEVELOPMENT, V128, P2095

[10] Male-to-female sex reversal in mice lacking fibroblast growth factor 9 [J].

Colvin, JS ;

Green, RP ;

Schmahl, J ;

Capel, B ;

Ornitz, DM .

CELL, 2001, 104 (06) :875-889

← 1 2 3 4 5 →