Gastric Cancer Cell-Derived Exosomes Can Regulate the Biological Functions of Mesenchymal Stem Cells by Inducing the Expression of Circular RNA circ_0004303

被引：24

作者：

Ba, Li ^{[1
]}

Xue, Chunling ^{[1
]}

Li, Xuechun ^{[1
]}

Zhang, Mingjia ^{[1
]}

Yang, Ying ^{[2
]}

Han, Qin ^{[1
]}

Sun, Zhao ^{[2
]}

Zhao, Robert Chunhua ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Inst Basic Med Sci, Sch Basic Med,Ctr Excellence Tissue Engn,Beijing, Peking Union Med Coll,Peking Union Med Coll Hosp, 5 Dongdan San Tiao Donghuamen St, Beijing 100005, Peoples R China

[2] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Oncol, Beijing, Peoples R China

来源：

STEM CELLS AND DEVELOPMENT | 2021年 / 30卷 / 16期

基金：

中国国家自然科学基金;

关键词：

tumor microenvironment; mesenchymal stem cells; exosomes; circular RNAs; ADHESION MOLECULE ALCAM; STROMAL CELLS; METASTASIS; MIGRATION; INVASION;

D O I：

10.1089/scd.2021.0059

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

As an important component of the dynamic tumor microenvironment, mesenchymal stem cells (MSCs) can interact with tumor cells to promote tumor growth. Treatment with tumor cell-derived exosomes can change the biological functions of MSCs. We want to study the mechanism by which exosomes derived from gastric cancer cells affect the biological functions of MSCs. After MSCs were treated with adenocarcinoma gastric cells (AGS) cell-derived exosomes, circular RNAs differentially expressed in MSCs were verified using existing RNA microarray results combined with quantitative real-time polymerase chain reaction (qRT-PCR). Then, circular RNAs were knocked down or overexpressed by plasmids, and the functions of circular RNAs were evaluated by Migration and invasion assay. Dual luciferase reporter assay was used to evaluate the potential mechanism of circular RNAs. After treatment with exosomes secreted by AGS, the results showed that some circular RNAs expressed by human adipose-derived MSCs showed significant differences. The elevated circ_0004303 promoted the migration and invasion of human adipose-derived MSCs in vitro. Circ_0004303 upregulated the expression of activated leukocyte cell adhesion molecule (ALCAM) by acting as a miR-148a-3p sponge, thereby enhancing the migration and invasion functions of human adipose-derived MSCs. Therefore, exosomes secreted by AGS can affect the expression of circular RNAs in human adipose-derived MSCs. Hsa_circ_0004303 can regulate the migration and invasion of human adipose-derived MSCs via the miR-148a-3P/ALCAM axis. This study suggests that tumor cells can promote the migration and homing of MSCs in adjacent tissues by secreting exosomes.

引用

页码：830 / 842

页数：13

共 34 条

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