The normal trachea is cleaned by MUC5B mucin bundles from the submucosal glands coated with the MUC5AC mucin

被引：91

作者：

Ermund, Anna ^{[1
]}

Meiss, Lauren N. ^{[1
]}

Rodriguez-Pineiro, Ana M. ^{[1
]}

Baehr, Andrea ^{[2
]}

Nilsson, Harriet E. ^{[1
,3
,4
]}

Trillo-Muyo, Sergio ^{[1
]}

Ridley, Caroline ^{[5
]}

Thornton, David J. ^{[5
]}

Wine, Jeffrey J. ^{[6
]}

Hebert, Hans ^{[3
,4
]}

Klymiuk, Nikolai ^{[2
]}

Hansson, Gunnar C. ^{[1
]}

机构：

[1] Univ Gothenburg, Dept Med Biochem, Box 440, SE-40530 Gothenburg, Sweden

[2] Ludwig Maximilians Univ Munchen, Inst Mol Anim Breeding & Biotechnol, Gene Ctr, Feodor Lynen Str 25, D-81377 Munich, Germany

[3] KTH Royal Inst Technol, Dept Biosci & Nutr, Karolinska Inst, SE-14157 Huddinge, Sweden

[4] KTH Royal Inst Technol, Sch Technol & Hlth, SE-14157 Huddinge, Sweden

[5] Univ Manchester, Wellcome Trust Ctr Cell Matrix Res, Fac Biol Med & Hlth, Manchester, Lancs, England

[6] Stanford Univ, Cyst Fibrosis Res Lab, Stanford, CA 94305 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 492卷 / 03期

基金：

瑞典研究理事会;

关键词：

Respiratory tract; Mucus; MUC5AC; Airway surface liquid; Lung; CYSTIC-FIBROSIS; MUCOCILIARY TRANSPORT; MUCUS; DOMAIN; AIRWAY; MODEL;

D O I：

10.1016/j.bbrc.2017.08.113

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To understand the mucociliary clearance system, mucins were visualized by light, confocal and electron microscopy, and mucus was stained by Alcian blue and tracked by video microscopy on tracheal explants of newborn piglets. We observed long linear mucus bundles that appeared at the submucosal gland openings and were transported cephalically. The mucus bundles were shown by mass spectrometry and immunostaining to have a core made of MUC5B mucin and were coated with MUC5AC mucin produced by surface goblet cells. The transport speed of the bundles was slower than the airway surface liquid flow. We suggest that the goblet cell MUC5AC mucin anchors the mucus bundles and thus controls their transport. Normal clearance of the respiratory tree of pigs and humans, both rich in submucosal glands, is performed by thick and long mucus bundles. (C) 2017 The Authors. Published by Elsevier Inc.

引用

页码：331 / 337

页数：7

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