Application of several types of substrates to ficin-catalyzed peptide synthesis

被引：11

作者：

Sekizaki, H. ^{[1
]}

Toyota, E. ^{[1
]}

Fuchise, T. ^{[1
]}

Zhou, S. ^{[2
]}

Noguchi, Y. ^{[1
]}

Horita, K. ^{[1
]}

机构：

[1] Hlth Sci Univ Hokkaido, Fac Pharmaceut Sci, Ishikari, Hokkaido 0610293, Japan

[2] Qingdao Univ, Coll Med, Dept Pharm, Qingdao, Peoples R China

来源：

AMINO ACIDS | 2008年 / 34卷 / 01期

关键词：

N-Boc-amino acid; amidinophenyl esters; guanidinophenylesters; (guanidinomethyl) phenyl ester; guanidinonaphthyl ester; enzymatic peptide synthesis; ficin;

D O I：

10.1007/s00726-006-0489-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The capability of ficin, a cystine protease, to form peptide bonds was investigated using several types of N-Boc-amino acid phenyl and naphthyl esters as acyl donor components. Enzyme-catalyzed peptide synthesis was carried out under optimized reaction conditions of pH, acyl acceptor concentration and selection of the best yield organic solvent. It used a condensation of N-Boc-Ala-OpGu and Ala-pNA as a model reaction. The products were obtained in 72-96% yield using 10 different substrates, within a few minutes of reaction time.

引用

页码：149 / 153

页数：5

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