PHYTOCHEMICAL ANALYSIS OF HYDROETHANOLIC EXTRACTS FROM POWDERED ROOTS OF Panax ginseng C. A. Meyer AND Heteropterys tomentosa A. Juss AND EVALUATION OF THEIR EFFECTS ON ASTROCYTE CELL DEATH

被引:5
作者
Bezerra, Andreia Gomes [1 ]
Negri, Giuseppina [1 ,4 ]
Duarte-Almeida, Joaquim Mauricio [2 ,4 ]
Smaili, Soraya Soubhi [3 ]
Carlini, Elisaldo Araujo [4 ]
机构
[1] Univ Fed Sao Paulo, Dept Psicobiol, BR-04023062 Sao Paulo, SP, Brazil
[2] Univ Fed Sao Joao del Rei, Lab Farmacognosia & Prod Nat, BR-35501296 Divinopolis, MG, Brazil
[3] Univ Fed Sao Paulo, Dept Farmacol, BR-04044020 Sao Paulo, SP, Brazil
[4] Univ Fed Sao Paulo, Dept Med Prevent, BR-04023062 Sao Paulo, SP, Brazil
来源
QUIMICA NOVA | 2016年 / 39卷 / 05期
基金
巴西圣保罗研究基金会;
关键词
neuroprotection; toxicity; ginsenosides; caffeoylquinic acid derivatives; astilbin isomers; PERFORMANCE LIQUID-CHROMATOGRAPHY; FLIGHT MASS-SPECTROMETRY; QIONG-YU-GAO; OXIDATIVE STRESS; BRAZILIAN PLANTS; HOLISTIC QUALITY; APOPTOSIS; IDENTIFICATION; GINSENOSIDES; SAPONINS;
D O I
10.5935/0100-4042.20160069
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The medicinal plants Panax ginseng C. A. Meyer (Araliaceae) and Heteropterys tomentosa A. Juss (Malpighiaceae) are widely and separately used by the Brazilian population as phytotherapeutics for the same medicinal purposes as tonics and to improve cognition. A chemical analysis was carried out on hydroethanolic extracts of powdered roots from P. ginseng and H. tomentosa using HPLC-DAD-ESI-MS/MS (High Performance Liquid Chromatography coupled to Diode-Array Detector and Electrospray Ionization-Mass Spectrum/Mass Spectrum). The ginsenosides Rg1, Rf, mRg and mRf were the main constituents in a hydroethanolic extract from P. ginseng, while in the hydroethanolic extract from H. tomentosa, caffeoylquinic acid derivatives and astilbin isomers were the main constituents. Concentration-time-effect curves were generated in cultures of astrocytes that were incubated with hydroethanolic extracts of these species to elucidate their toxicities. The P. ginseng extract was nontoxic at all of the tested times and concentrations. The hydroethanolic extract from H. tomentosa demonstrated toxicity at a concentration of 1000 mu g/mL. P. ginseng extract had no protective effect against staurosporine. Many studies have demonstrated the neuroprotective effect of ginsenosides, caffeoylquinic derivatives and flavonoids.
引用
收藏
页码:581 / 587
页数:7
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