Protoplasts in the analysis of early plant-pathogen interactions: current applications and perspectives

被引：0

作者：

Xing, Tim ^{[1
]}

Li, Xiu-Qing ^{[2
]}

Laroche, Andre ^{[3
]}

Tian, Lining ^{[4
]}

Tubei, Kipkios ^{[1
]}

Wang, Xiaojing ^{[5
]}

机构：

[1] Carleton Univ, Dept Biol, Ottawa, ON, Canada

[2] Agr & Agri Food Canada, Fredericton Res & Dev Ctr, Fredericton, NB, Canada

[3] Agr & Agri Food Canada, Lethbridge Res & Dev Ctr, Lethbridge, AB, Canada

[4] Agr & Agri Food Canada, London Res & Dev Ctr, London, ON, Canada

[5] South China Normal Univ, Coll Life Sci, Guangdong Key Lab Biotechnol Plant Dev, Guangzhou 510631, Guangdong, Peoples R China

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2017年 / 149卷 / 04期

基金：

中国国家自然科学基金; 加拿大自然科学与工程研究理事会;

关键词：

Protoplast; Effector; Elicitor; R gene; Signal perception; Transduction; Transient gene expression; Protein-protein interaction; Disease resistance; PROGRAMMED CELL-DEATH; TRANSIENT EXPRESSION; GENE-EXPRESSION; PROTEIN; RESISTANCE; DEFENSE; SYSTEM; WALL; ELICITOR; IMMUNITY;

D O I：

10.1007/s10658-017-1230-9

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Transient gene expression in protoplasts has been applied to the analysis of gene expression, promoter activity, subcellular localization of proteins, and genome editing. Its advantages have been realized in recent years in various physiological, biochemical, genetic, and molecular biological studies. The omics and systems biology studies of plant development and their interactions with the environment have revitalized this approach. Early plant-pathogen interaction represents one of the most unique signalling research areas. Here, we try to discuss some developments and current applications of the protoplast system. It is for sure that the system will significantly bridge the gap between high throughput approaches and functional analysis in the study of plant-pathogen interactions in the omics era.

引用

页码：1001 / 1010

页数：10

共 69 条

[1] The Xanthomonas Ax21 protein is processed by the general secretory system and is secreted in association with outer membrane vesicles [J].