Identification of Differentially Expressed Genes in Resistant Tetraploid Wheat (Triticum turgidum) under Sitobion avenae (F.) Infestation

被引：0
作者：
Liu, Xinlun [1 ,2 ]
Kou, Xudan [1 ,2 ]
Bai, Shichao [1 ,2 ]
Luo, Yufeng [1 ,2 ]
Wang, Zhenyu [1 ,2 ]
Xie, Lincai [1 ,2 ]
Deng, Pingchuan [1 ,2 ]
Zhang, Hong [1 ,2 ]
Wang, Changyou [1 ,2 ]
Wang, Yajuan [1 ,2 ]
Zhao, Jixin [1 ,2 ]
Ji, Wanquan [1 ,2 ]
机构：
[1] Northwest A&F Univ, Coll Agron, State Key Lab Crop Stress Biol Arid Areas, Xianyang 712100, Peoples R China
[2] Minist Agr, Shaanxi Res Stn Crop Gene Resources & Germplasm E, Xianyang 712100, Peoples R China
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 11期
关键词：
aphid resistance; tetraploid wheat; Sitobion avenae; suppression subtractive hybridization; gene expression; ENGLISH GRAIN APHID; DEFENSE RESPONSES; DIURAPHIS-NOXIA; BREAD WHEAT; TOLERANCE; PHLOEM; TIME; INSECTICIDE; AESTIVUM; BARLEY;
D O I：
10.3390/ijms23116012
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The grain aphid Sitobion avenae (Fabricius) is one of the most destructive pests of wheat (Triticum aestivum). Deployment of resistant wheat germplasm appears as an excellent solution for this problem. Elite bread wheat cultivars only have limited resistance to this pest. The present study was carried out to investigate the potential of the tetraploid wheat (Triticum turgidum) variety Lanmai, which showed high resistance to S. avenae at both seedling and adult plant stages, as a source of resistance genes. Based on apterous adult aphids' fecundity tests and choice bioassays, Lanmai has been shown to display antixenosis and antibiosis. Suppression subtractive hybridization (SSH) was employed to identify and isolate the putative candidate defense genes in Lanmai against S. avenae infestation. A total of 134 expressed sequence tags (ESTs) were identified and categorized based on their putative functions. RT-qPCR analysis of 30 selected genes confirmed their differential expression over time between the resistant wheat variety Lanmai and susceptible wheat variety Polan305 during S. avenae infestation. There were 11 genes related to the photosynthesis process, and only 3 genes showed higher expression in Lanmai than in Polan305 after S. avenae infestation. Gene expression analysis also revealed that Lanmai played a critical role in salicylic acid and jasmonic acid pathways after S. avenae infestation. This study provided further insights into the role of defense signaling networks in wheat resistance to S. avenae and indicates that the resistant tetraploid wheat variety Lanmai may provide a valuable resource for aphid tolerance improvement in wheat.
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共 61 条
  • [1] Searching for wheat resistance to aphids and wheat bulb fly in the historical Watkins and Gediflux wheat collections
    Aradottir, G. I.
    Martin, J. L.
    Clark, S. J.
    Pickett, J. A.
    Smart, L. E.
    [J]. ANNALS OF APPLIED BIOLOGY, 2017, 170 (02) : 179 - 188
  • [2] Inheritance of Russian wheat aphid resistance from tetraploid wheat accessions during transfer to hexaploid wheat
    Beyer, Benjamin M.
    Haley, Scott D.
    Lapitan, Nora L. V.
    Peng, Junhua H.
    Peairs, Frank B.
    [J]. EUPHYTICA, 2011, 179 (02) : 247 - 255
  • [3] Near-isogenic lines of Triticum aestivum with distinct modes of resistance exhibit dissimilar transcriptional regulation during Diuraphis noxia feeding
    Botha, Anna-Maria
    van Eck, Leon
    Burger, N. Francois V.
    Swanevelder, Zacharias H.
    [J]. BIOLOGY OPEN, 2014, 3 (11): : 1116 - 1126
  • [4] Transcript Profiling of Wheat Genes Expressed During Feeding by Two Different Biotypes of Diuraphis noxia
    Botha, Anna-Maria
    Swanevelder, Zacharias H.
    Lapitan, Nora L. V.
    [J]. ENVIRONMENTAL ENTOMOLOGY, 2010, 39 (04) : 1206 - 1231
  • [5] Boyko EV, 2006, J ECON ENTOMOL, V99, P1430, DOI 10.1603/0022-0493-99.4.1430
  • [6] Proteinase inhibitor accumulation in aphid-infested barley leaves
    Casaretto, JA
    Corcuera, LJ
    [J]. PHYTOCHEMISTRY, 1998, 49 (08) : 2279 - 2286
  • [7] Exploring the metabolic variation between domesticated and wild tetraploid wheat genotypes in response to corn leaf aphid infestation
    Chandrasekhar, K.
    Shavit, R.
    Distelfeld, A.
    Christensen, S. A.
    Tzin, V.
    [J]. PLANT SIGNALING & BEHAVIOR, 2018, 13 (06)
  • [8] Blast2GO:: a universal tool for annotation, visualization and analysis in functional genomics research
    Conesa, A
    Götz, S
    García-Gómez, JM
    Terol, J
    Talón, M
    Robles, M
    [J]. BIOINFORMATICS, 2005, 21 (18) : 3674 - 3676
  • [9] Seasonal and annual genotypic variation and the effect of climate on population genetic structure of the cereal aphid Sitobion avenae in northern France
    Dedryver, C. -A.
    Le Gallic, J. -F.
    Haack, L.
    Halkett, F.
    Outreman, Y.
    Simon, J. -C.
    [J]. BULLETIN OF ENTOMOLOGICAL RESEARCH, 2008, 98 (02) : 159 - 168
  • [10] Microarray analysis of the interaction between the aphid Rhopalosiphum padi and host plants reveals both differences and similarities between susceptible and partially resistant barley lines
    Delp, Gabriele
    Gradin, Therese
    Ahman, Inger
    Jonsson, Lisbeth M. V.
    [J]. MOLECULAR GENETICS AND GENOMICS, 2009, 281 (03) : 233 - 248
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