Magnetic Amine-Functionalized UiO-66 for Oxaliplatin Delivery to Colon Cancer Cells: In Vitro Studies

UiO-66-NH2 (U) and its magnetic UiO-66-NH2 form (MU) were used to enhance Oxaliplatin (OX) efficacy. The fundamental physical and structural properties of nanoparticles were characterized via X-ray Diffraction (XRD), Fourier Transform Infrared (FTIR), UV-Visible (UV-Vis), Brunauer-Emmett-Teller (BET) surface area analysis, field emission scanning electron microscopy (FESEM), Energy-dispersive X-ray analysis (EDAX) and mapping, and Dynamic light scattering (DLS). The results further showed an improved anticancer activity and efficacy of the designed drug delivery systems (DDSs) compared to OX in 2-and 3-dimensional models of colorectal cancer in which cell viability, proliferation and migration, and morphology were assessed. Additionally, the oxidative/antioxidant activity of U and MU-loaded OX demonstrated greater oxidative behavior compared to OX. In vitro experiments showed that U and MU could inhibit the proliferation and migration of colorectal cancer cells in a dose-dependent manner. The half-maximal inhibitory concentration (IC50) values were found to be 6.10 ppm for OX, 18.47 ppm for MU(OX), and 47.02 ppm for U(OX), respectively. The U(OX) and MU(OX) were more effective than OX in terms of drug release. The results indicate that U and MU show considerable promise as a novel and effective drug delivery system for the treatment of colorectal cancer and possibly other malignancies.