Response surface methodology-based optimization of glucose acylation bio-catalyzed by immobilized lipase

被引:3
|
作者
Choudhry, Ayoub Rashid [1 ]
Mushtaq, Muhammad [1 ]
Adnan, Ahmad [1 ]
Syed, Quratulain [2 ]
机构
[1] Govt Coll Univ, Dept Chem, Lahore, Pakistan
[2] Pakistan Council Sci & Ind Res, Lahore, Pakistan
关键词
Biocatalyst; immobilization; optimization; acylation; glucose monolaurate; CANDIDA-ANTARCTICA LIPASE; ENZYME IMMOBILIZATION; INTERFACIAL ACTIVATION; ORGANIC-SOLVENTS; BIOCATALYSIS; SELECTIVITY; ACID; STABILIZATION; MODULATION; STABILITY;
D O I
10.1080/10242422.2017.1320991
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This paper describes in detail the selection and optimization of immobilized lipases for enhanced regioselective acylation of glucose into glucose monolaurate (GlcML). Initially, nature of biocatalyst, immobilization approach, reaction media, glucose, and lauric acid concentration were screened out. Finally, lipases from Rhizopus arrhizus immobilized on dead mycelia were investigated under various reaction conditions (Temperature, shaking speed, enzyme dose, and water content) following a fully rotatable central composite design (FRCCD) to optimize the activity of lipases. The immobilized lipases-based biocatalysts in the presence of polar solvents (tertiary alcohols) and higher concentrations of substrates i. e. glucose and lauric acid (100 and 300mmol L-1, respectively) offered conversion rate of 1.5 mmolmin(-1) L-1. Moreover, optimization of reaction conditions revealed that 162.5 lipase units/100mL at 31.25 degrees C, 3% water content, and 105RPM shaking speed enhanced the conversion rate by 0.5 mmolmin(-1) L-1 rendering the reaction more economical. Hence, lipases-based immobilized biocatalysts may provide an intelligent and green choice for commercial scale synthesis of GlcML for food and pharmaceutical industries.
引用
收藏
页码:238 / 248
页数:11
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