Covalent and selective immobilization of fusion proteins

被引：127

作者：

Kindermann, M

George, N

Johnsson, N

Johnsson, K ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, Inst Mol & Biol Chem, CH-1015 Lausanne, Switzerland

[2] Swiss Fed Inst Technol, Inst Biomol Sci, CH-1015 Lausanne, Switzerland

[3] Forschungszentrum Karlsruhe, Inst Toxicol & Genet, D-76021 Karlsruhe, Germany

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 26期

关键词：

D O I：

10.1021/ja034145s

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A general method for the covalent immobilization of fusion proteins is presented. The approach is based on the unusual mechanism of the human O6-alkylguanine-DNA alkyltransferase, which irreversibly transfers the alkyl group from its substrate, alkylated or benzylated guanine, to a reactive cysteine residue. By attaching the benzyl group to a surface, hAGT fusion proteins immobilize themselves in a specific and covalent manner. The specificity of the reaction of hAGT with its substrate even allows the specific immobilization of hAGT fusion proteins directly out of cell extracts, making the approach an attractive alternative to currently used immobilization procedures. Copyright © 2003 American Chemical Society.

引用

页码：7810 / 7811

页数：2

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