PI3 kinase-dependent stimulation of platelet migration by stromal cell-derived factor 1 (SDF-1)

被引：75

作者：

Kraemer, Bjoern F. ^{[2
]}

Borst, Oliver ^{[1
,2
]}

Gehring, Eva-Maria ^{[1
]}

Schoenberger, Tanja ^{[2
]}

Urban, Benjamin ^{[2
]}

Ninci, Elena ^{[2
]}

Seizer, Peter ^{[2
]}

Schmidt, Christine ^{[2
]}

Bigalke, Boris ^{[2
]}

Koch, Miriam ^{[2
]}

Martinovic, Ivo ^{[3
]}

Daub, Karin ^{[2
]}

Merz, Tobias ^{[4
]}

Schwanitz, Laura ^{[2
]}

Stellos, Konstantinos ^{[2
]}

Fiesel, Fabienne ^{[5
]}

Schaller, Martin ^{[6
]}

Lang, Florian ^{[1
]}

Gawaz, Meinrad ^{[2
]}

Lindemann, Stephan ^{[2
]}

机构：

[1] Univ Tubingen, Inst Physiol, D-72076 Tubingen, Germany

[2] Univ Klinikum Tubingen, Med Klin, Abt 3, D-72076 Tubingen, Germany

[3] Univ Marburg, Klin Herz & Thorakale Gefasschirurg, D-35037 Marburg, Germany

[4] Univ Tubingen, Nat Wissensch & Med Inst, D-72770 Reutlingen, Germany

[5] HERTIE Inst Klin Hirnforsch, Abt Neurodegenerat Erkrankungen, D-72076 Tubingen, Germany

[6] Univ Hautklin Tubingen, Haut Klin, D-72076 Tubingen, Germany

来源：

JOURNAL OF MOLECULAR MEDICINE-JMM | 2010年 / 88卷 / 12期

关键词：

Platelets; SDF-1; Cell migration; Cell signaling; Inflammation; ENDOTHELIAL PROGENITOR CELLS; TRANSENDOTHELIAL MIGRATION; PERTUSSIS-TOXIN; T-LYMPHOCYTES; CD34(+) CELLS; CHEMOKINE; ACTIVATION; CHEMOTAXIS; RECEPTORS; CXCR4;

D O I：

10.1007/s00109-010-0680-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Platelets have been regarded as static cells that do not move once they adhere to a matrix. The present study explored, whether platelets are able to migrate. In contrast to the current opinion, we found that platelets were mobile, able to migrate over a surface, and transmigrate through a transwell membrane and endothelium toward a source of stromal cell-derived factor 1 (SDF-1). Platelet migration was stimulated by SDF-1, which led to the downstream activation and phosphorylation of Wiskott-Aldrich syndrome protein. SDF-1 signaling and subsequent platelet migration could be inhibited by CXCR4-receptor blocker AMD3100, pertussis toxin, inhibition of phosphoinositol 3-kinase (PI3 kinase) with LY294002 or wortmannin, and disruption of actin polymerization with cytochalasin B. The potential of platelets to migrate in an SDF-1-mediated fashion may redefine the role of platelets in the pathophysiology of vascular inflammation, subsequent atherosclerotic degeneration, and vascular regeneration.

引用

页码：1277 / 1288

页数：12

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