Magnetic cation exchange isolation of lysozyme from native hen egg white

被引：0

作者：

Safarik, Ivo ^{[1
,2
]}

Sabatkova, Zdenka ^{[1
]}

Tokar, Oldrich ^{[3
]}

Safarikova, Mirka ^{[1
]}

机构：

[1] Acad Sci Czech Republic, Inst Syst Biol & Ecol, Dept Biomagnet Tech, CZ-37005 Ceske Budejovice, Czech Republic

[2] Univ S Bohemia, Fac Biol Sci, Dept Gen Biol, CZ-37005 Ceske Budejovice, Czech Republic

[3] Iontosorb, CZ-40007 Usti Nad Labem, Czech Republic

来源：

FOOD TECHNOLOGY AND BIOTECHNOLOGY | 2007年 / 45卷 / 04期

关键词：

hen egg white; lysozyme; magnetic separation; cellulose cation exchangers;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two magnetic macroporous, cellulose cation exchangers (Iontosorb MG CM 100 and Iontosorb MG SHP 100) were used for one-step isolation of lysozyme from native, undiluted hen egg white. Highly purified lysozyme (purity >96%) with specific activity similar to that of commercial lysozyme preparations was obtained in both cases. Carboxymethyl-based cation exchanger exhibited substantially higher capacity for lysozyme; maximum adsorption capacity was 138 mg/mL. The lysozyme-depleted egg white can be used in the same way as the routinely used egg white because no dilution of this material was necessary during the purification process.

引用

页码：355 / 359

页数：5

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