Hematopoietic reconstitution of CD34+ cells grown in static and stirred culture systems in NOD/SCID mice

被引：10

作者：

Yang, Shi ^{[1
]}

Cai, Haibo ^{[1
]}

Jin, Huili ^{[1
]}

Tan, Wen-Song ^{[1
]}

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 01期

关键词：

CD34(+) cells; ex vivo expansion; NOD; SCID mice; static culture; stirred culture;

D O I：

10.1007/s10529-007-9517-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The hematopoietic reconstitution of cord blood (CB) CD34(+) cells grown in static and stirred system was studied. Static cultures were better than stirred cultures for cell expansion. Engraftment of stirred-culture hematopoietic stem cells (HSCs) was higher than static-culture HSCs. Stirred-culture HSCs had better multilineage reconstitution ability and colony-forming ability than static-culture HSCs. Static cultures thus favor the expansion of HSCs and stirred cultures are more effective in preserving functional HSCs.

引用

页码：61 / 65

页数：5

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