Measuring the effects of postmortem time and age on mouse lens elasticity using atomic force microscopy

被引：4

作者：

Batchelor, Wyndham More ^{[1
]}

Heilman, Bianca Maceo ^{[1
,2
]}

Arrieta-Quintero, Esdras ^{[2
]}

Ruggeri, Marco ^{[2
]}

Parel, Jean-Marie ^{[1
,2
,3
]}

Manns, Fabrice ^{[1
,2
]}

Cabrera-Ghayouri, Sara ^{[4
]}

Dibas, Mohammed ^{[4
]}

Ziebarth, Noel Marysa ^{[1
,2
]}

机构：

[1] Univ Miami, Coll Engn, Dept Biomed Engn, Biomed Atom Force Microscopy Lab, Miami, FL USA

[2] Univ Miami, Miller Sch Med, Bascom Palmer Eye Inst, Ophthalm Biophys Ctr, Miami, FL 33136 USA

[3] Univ New South Wales, Brien Holden Vis Inst, Sydney, NSW, Australia

[4] AbbVie, Irvine, CA USA

来源：

EXPERIMENTAL EYE RESEARCH | 2021年 / 212卷

关键词：

Lens; Presbyopia; Biomechanics; Atomic force microscopy; Postmortem time; Incubation time; Age; CRYSTALLINE LENS; STIFFNESS; CATARACT; BIOMETRY; PROTEIN; MICE; EYE;

D O I：

10.1016/j.exer.2021.108768

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

The mouse lens is frequently used both in vivo and ex vivo in ophthalmic research to model conditions affecting the human lens, such as presbyopia. The mouse lens has a delicate structure which is prone to damage and biomechanical changes both before and after extraction from the whole globe. When not properly controlled for, these changes can confound the biomechanical analysis of mouse lenses. In this study, atomic force microscopy microindentation was used to assess changes in the Young's Modulus of Elasticity of the mouse lens as a function of mouse age and postmortem time. Old mouse lenses measured immediately postmortem were significantly stiffer than young mouse lenses (p = 0.028). However, after 18 h of incubation, there was no measurable difference in lens stiffness between old and young mouse lenses (p = 0.997). This demonstrates the need for careful experimental control in experiments using the mouse lens, especially regarding postmortem time.

引用

页数：6

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