A Spatiotemporal DNA Endoploidy Map of the Arabidopsis Root Reveals Roles for the Endocycle in Root Development and Stress Adaptation

被引：70

作者：

Bhosale, Rahul ^{[1
,2
,3
,4
]}

Boudolf, Veronique ^{[1
,2
]}

Cuevas, Fabiola ^{[1
,2
]}

Lu, Ran ^{[1
,2
]}

Eekhout, Thomas ^{[1
,2
]}

Hu, Zhubing ^{[1
,2
,5
]}

Van Isterdael, Gert ^{[1
,2
,6
]}

Lambert, Georgina M. ^{[7
]}

Xu, Fan ^{[8
]}

Nowack, Moritz K. ^{[1
,2
]}

Smith, Richard S. ^{[9
]}

Vercauteren, Ilse ^{[1
,2
]}

De Rycke, Riet ^{[1
,2
,10
]}

Storme, Veronique ^{[1
,2
]}

Beeckman, Tom ^{[1
,2
]}

Larkin, John C. ^{[11
]}

Kremer, Anna ^{[4
,10
]}

Hofte, Herman ^{[8
]}

Galbraith, David W. ^{[7
,12
]}

Kumpf, Robert P. ^{[1
,2
]}

Maere, Steven ^{[1
,2
,3
]}

De Veylder, Lieven ^{[1
,2
]}

机构：

[1] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium

[2] VIB Ctr Plant Syst Biol, B-9052 Ghent, Belgium

[3] Univ Ghent, Bioinformat Inst Ghent, B-9052 Ghent, Belgium

[4] Univ Nottingham, Ctr Plant Integrat Biol, Loughborough LE12 5RD, England

[5] Henan Univ, Inst Plant Stress Biol, Dept Biol, State Key Lab Cotton Biol, Kaifeng 475004, Peoples R China

[6] VIB Flow Core, B-9052 Ghent, Belgium

[7] Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA

[8] Univ Paris Saclay, CNRS, AgroParisTech, Inst Jean Pierre Bourgin,INRA, F-78000 Versailles, France

[9] Max Planck Inst Plant Breeding Res, Dept Comparat Dev & Genet, D-50829 Cologne, Germany

[10] VIB Bio Imaging Core, B-9052 Ghent, Belgium

[11] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA

[12] Henan Univ, Sch Life Sci, State Key Lab Cotton Biol, Key Lab Plant Stress Biol, Kaifeng 475004, Peoples R China

来源：

PLANT CELL | 2018年 / 30卷 / 10期

基金：

美国国家科学基金会;

关键词：

DEPENDENT KINASE INHIBITORS; GENE-EXPRESSION; CELL-SIZE; TRANSCRIPTION FACTOR; HAIR INITIATION; O-ACETYLATION; ENDOREDUPLICATION; GROWTH; THALIANA; CYCLE;

D O I：

10.1105/tpc.17.00983

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Somatic polyploidy caused by endoreplication is observed in arthropods, molluscs, and vertebrates but is especially prominent in higher plants, where it has been postulated to be essential for cell growth and fate maintenance. However, a comprehensive understanding of the physiological significance of plant endopolyploidy has remained elusive. Here, we modeled and experimentally verified a high-resolution DNA endoploidy map of the developing Arabidopsis thaliana root, revealing a remarkable spatiotemporal control of DNA endoploidy levels across tissues. Fitting of a simplified model to publicly available data sets profiling root gene expression under various environmental stress conditions suggested that this root endoploidy patterning may be stress-responsive. Furthermore, cellular and transcriptomic analyses revealed that inhibition of endoreplication onset alters the nuclear-to-cellular volume ratio and the expression of cell wall-modifying genes, in correlation with the appearance of cell structural changes. Our data indicate that endopolyploidy might serve to coordinate cell expansion with structural stability and that spatiotemporal endoreplication pattern changes may buffer for stress conditions, which may explain the widespread occurrence of the endocycle in plant species growing in extreme or variable environments.

引用

页码：2330 / 2351

页数：22

共 99 条

[1] WATER-DEFICIT IN DEVELOPING ENDOSPERM OF MAIZE - CELL-DIVISION AND NUCLEAR-DNA ENDOREDUPLICATION [J].