Rapid and Sensitive SERS-Based Lateral Flow Test for SARS-CoV2-Specific IgM/IgG Antibodies

被引：57

作者：

Srivastav, Supriya ^{[1
,2
]}

Dankov, Asen ^{[1
,2
]}

Adanalic, Mujo ^{[1
,2
]}

Grzeschik, Roland ^{[1
,2
]}

Vi Tran ^{[1
,2
]}

Pagel-Wieder, Sibylle ^{[3
]}

Gessler, Frank ^{[3
]}

Spreitzer, Ingo ^{[4
]}

Scholz, Tatjana ^{[5
]}

Schnierle, Barbara ^{[5
]}

Anastasiou, Olympia E. ^{[6
]}

Dittmer, Ulf ^{[6
]}

Schluecker, Sebastian ^{[1
,2
]}

机构：

[1] Univ Duisburg Essen, Dept Chem, D-45141 Essen, Germany

[2] Univ Duisburg Essen, CENIDE, D-45141 Essen, Germany

[3] Miprolab Gesell Mikrobiolog Diagnost MbH, D-37079 Gottingen, Germany

[4] Paul Ehrlich Inst, Dept Microbiol, D-63225 Langen, Germany

[5] Paul Ehrlich Inst, Dept Virol, D-63225 Langen, Germany

[6] Univ Duisburg Essen, Univ Clin Essen, Inst Virol, D-45147 Essen, Germany

来源：

ANALYTICAL CHEMISTRY | 2021年 / 93卷 / 36期

关键词：

IMMUNOCHROMATOGRAPHIC ASSAY; QUANTITATIVE DETECTION; IGG;

D O I：

10.1021/acs.analchem.1c02305

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

As an immune response to COVID-19 infection, patients develop SARS-CoV-2-specific IgM/IgG antibodies. Here, we compare the performance of a conventional lateral flow assay (LFA) with a surface-enhanced Raman scattering (SERS)-based LFA test for the detection of SARS-CoV-2-specific IgM/IgG in sera of COVID-19 patients. Sensitive detection of IgM might enable early serological diagnosis of acute infections. Rapid detection in serum using a custom-built SERS reader is at least an order of magnitude more sensitive than the conventional LFAs with naked-eye detection. For absolute quantification and the determination of the limit of detection (LOD), a set of reference measurements using purified (total) IgM in buffer was performed. In this purified system, the sensitivity of SERS detection is even 7 orders of magnitude higher: the LOD for SERS was ca. 100 mu g/mL compared to ca. 1 mu g/mL for the naked-eye detection. This outlines the high potential of SERS-based LFAs in point-of-care testing once the interference of serum components with the gold conjugates and the nitrocellulose membrane is minimized.

引用

页码：12391 / 12399

页数：9

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