3,3′-Diindolylmethane increases bone mass by suppressing osteoclastic bone resorption in mice

被引:16
作者
Yu, Tai-yong [1 ,2 ]
Pang, Wei-jun [3 ]
Yang, Gong-she [3 ]
机构
[1] Ehime Univ, Grad Sch Med, Proteosci Ctr, Div Integrat Pathophysiol, Toon, Ehime 7910295, Japan
[2] Univ Tokyo, Inst Mol & Cellular Biosci, Lab Epigenet Skeletal Dis, Tokyo 1130032, Japan
[3] Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi Provinc, Peoples R China
基金
日本学术振兴会;
关键词
3,3 '-Diindolylmethane (DIM); Bone metabolism; Bone resorption; Osteoporosis; Ovariectomized (OVX); ARYL-HYDROCARBON RECEPTOR; PROSTATE-CANCER; DOWN-REGULATION; IN-VITRO; INDOLE-3-CARBINOL; ACTIVATION; APOPTOSIS; RESVERATROL; INHIBITION; INDUCTION;
D O I
10.1016/j.jphs.2014.11.006
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
3,3'-Diindolylmethane (DIM), a major acid-condensation product or metabolite of indole-3-carbinol which is found in cruciferous vegetables, has been shown to have anticancer, anti-inflammatory, and multiple immune stimulating effects. However, its function in bone metabolism is poorly understood. This study evaluated the effect of DIM on bone mass in mice under physiological and pathological conditions. Eight-week-old female mice received injections of a vehicle or 0.1 mg/g of DIM, twice a week for four weeks. We found that DIM treatment significantly increased bone mass as assessed by dual-energy X-ray absorptiometry (DEXA) and micro-computed tomography (ACT). Further, Bone histomorphometric analyses showed that this treatment significantly reduced bone resorption parameters, but did not increase bone formation parameters. Furthermore, we use ovariectomized (OVX)-induced osteoporotic mouse model, and explore function of DIM in skeletal pathological processes. Bone phenotype analyses revealed that the administration of DIM in this study effectively prevented OVX-induced bone loss resulting from increased bone resorption. Our results demonstrated that DIM increased bone mass by suppressing osteoclastic bone resorption in bone metabolism under both physiological and pathological conditions. Accordingly. DIM may be of value in the treatment and the possible prevention of bone diseases characterized by bone loss, such as postmenopausal osteoporosis. (C) 2014 Japanese Pharmacological Society. Production and hosting by Elsevier B.V.
引用
收藏
页码:75 / 82
页数:8
相关论文
共 55 条
  • [1] Molecular targets and anticancer potential of indole-3-carbinol and its derivatives
    Aggarwal, BB
    Ichikawa, H
    [J]. CELL CYCLE, 2005, 4 (09) : 1201 - 1215
  • [2] Influence of food polyphenols on aryl hydrocarbon receptor-signaling pathway estimated by in vitro bioassay
    Amakura, Yoshiaki
    Tsutsumi, Tomoaki
    Sasaki, Kumiko
    Nakamura, Masafumi
    Yoshida, Takashi
    Maitani, Tamio
    [J]. PHYTOCHEMISTRY, 2008, 69 (18) : 3117 - 3130
  • [3] RETRACTED: 3,3′-Diindolylmethane Enhances Chemosensitivity of Multiple Chemotherapeutic Agents in Pancreatic Cancer (Retracted article. See vol. 78, pg. 5467, 2018)
    Banerjee, Sanjeev
    Wang, Zhiwei
    Kong, Dejuan
    Sarkar, Fazlul H.
    [J]. CANCER RESEARCH, 2009, 69 (13) : 5592 - 5600
  • [4] RETRACTED: Down-regulation of androgen receptor by 3,3'-diindolylmethane contributes to inhibition of cell proliferation and induction of apoptosis in both hormone-sensitive LNCaP and insensitive C4-2B prostate cancer cells (Retracted article. See vol. 78, pg. 5473, 2018)
    Bhuiyan, Mohammad M. R.
    Li, Yiwei
    Banerjee, Sanjeev
    Ahmed, Fakhara
    Wang, Zhiwei
    Ali, Shadan
    Sarkar, Fazlul H.
    [J]. CANCER RESEARCH, 2006, 66 (20) : 10064 - 10072
  • [5] Guidelines for Assessment of Bone Microstructure in Rodents Using Micro-Computed Tomography
    Bouxsein, Mary L.
    Boyd, Stephen K.
    Christiansen, Blaine A.
    Guldberg, Robert E.
    Jepsen, Karl J.
    Mueller, Ralph
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 2010, 25 (07) : 1468 - 1486
  • [6] Casper RF, 1999, MOL PHARMACOL, V56, P784
  • [7] Indole-3-carbinol and diindolylmethane as aryl hydrocarbon (Ah) receptor agonists and antagonists in T47D human breast cancer cells
    Chen, I
    Safe, S
    Bjeldanes, L
    [J]. BIOCHEMICAL PHARMACOLOGY, 1996, 51 (08) : 1069 - 1076
  • [8] Cell Cycle-Dependent Effects of 3,3′-Diindolylmethane on Proliferation and Apoptosis of Prostate Cancer Cells
    Chinnakannu, Kannagi
    Chen, Di
    Li, Yiwei
    Wang, Zhiwei
    Dou, Q. Ping
    Reddy, G. Prem Veer
    Sarkar, Fazlul H.
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 2009, 219 (01) : 94 - 99
  • [9] Effect of curcumin on the aryl hydrocarbon receptor and cytochrome P450 1A1 in MCF-7 human breast carcinoma cells
    Ciolino, HP
    Daschner, PJ
    Wang, TTY
    Yeh, GC
    [J]. BIOCHEMICAL PHARMACOLOGY, 1998, 56 (02) : 197 - 206
  • [10] Prevention and treatment of bone metastases
    Coleman, Robert E.
    [J]. NATURE REVIEWS CLINICAL ONCOLOGY, 2012, 9 (02) : 76 - 78