Novel macrolactam compound produced by the heterologous expression of a large cryptic biosynthetic gene cluster of Streptomyces rochei IFO12908

被引：15

作者：

Hashimoto, Takuya ^{[1
]}

Kozone, Ikuko ^{[2
]}

Hashimoto, Junko ^{[2
]}

Ueoka, Reiko ^{[1
]}

Kagaya, Noritaka ^{[1
]}

Fujie, Manabu ^{[3
]}

Sato, Noriyuki ^{[3
]}

Ikeda, Haruo ^{[4
]}

Shin-ya, Kazuo ^{[1
,5
,6
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Koto Ku, 2-4-7 Aomi, Tokyo 1350064, Japan

[2] Japan Biol Informat Consortium, Koto Ku, 2-4-7 Aomi, Tokyo 1350064, Japan

[3] Okinawa Inst Sci & Technol Grad Univ, 1919-1 Tancha, Onna, Okinawa 9040495, Japan

[4] Kitasato Univ, Kitasato Inst Life Sci, Minami Ku, 1-15-1 Kitasato, Sagamihara, Kanagawa 2520373, Japan

[5] Univ Tokyo, Collaborat Res Inst Innovat Microbiol, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan

[6] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan

来源：

JOURNAL OF ANTIBIOTICS | 2020年 / 73卷 / 03期

关键词：

GENOME;

D O I：

10.1038/s41429-019-0265-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the course of our studies on the heterologous expression of giant biosynthetic genes, we discovered a novel cryptic biosynthetic gene cluster in Streptomyces rochei IFO12908. During our efforts to express biosynthetic genes using the host SUKA strain derived from Streptomyces avermitilis, a novel polyene macrolactam compound designated as JBIR-156 was produced. We report herein the cloning and heterologous expression of the JBIR-156 biosynthetic gene cluster, and the isolation, structure determination, and cytotoxic activity of this novel compound.

引用

页码：171 / 174

页数：4

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