Molecular characterization of Kluyveromyces marxianus strains isolated from agave fourcroydes (Lem.) in yucatan, Mexico

被引：12

作者：

Daisy, Perez-Brito

Raul, Tapia-Tussell

Quijano-Ramayo, Andres

Larque-Saavedra, Alfonso

Lappe, Patricia

机构：

[1] Ctr Invest Cient Yucatan, Lab GeMBio, Merida 97200, Yucatan, Mexico

[2] Univ Nacl Autonoma Mexico, Inst Biol, Mexico City 04510, DF, Mexico

来源：

MOLECULAR BIOTECHNOLOGY | 2007年 / 37卷 / 03期

关键词：

agave fourcroydes; Kluyveromyces; marxianus; AP-PCR analysis; PCR-RFLP analysis of rDNA; fingerprinting;

D O I：

10.1007/s12033-007-0036-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The molecular characterization of 14 strains of Kluyveromyces marxianus isolated from Agave fourcroydes (Lem.) in Yucatan, Mexico, was performed by AP-PCR analysis, PCR-RFLP of 5.8S-ITS, and complete NTS regions. A sequence analysis of the D1/D2 domain of the 26S rDNA was also carried out in six selected strains. The AP-PCR approach had the highest discrimination power for the molecular characterization of new henequen K. marxianus strains. PCR-RFLP of 5.8S-ITS regions did not reveal polymorphisms in this group of strains. The restriction enzyme digestion analysis of NTS region enables the separation among strains which coincides with ascospore shape groups. The molecular tools used in this article may be useful to confirm a preliminary screen of yeasts isolated from henequen without the use of growth characteristics or morpho-physiological tests.

引用

页码：181 / 186

页数：6

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