Recombinant protein secretion in Escherichia coli

被引：369
|
作者：
Mergulhao, FJM
Summers, DK
Monteiro, GA
机构：
[1] Inst Super Tecn, Ctr engn Biol & Quim, P-1049001 Lisbon, Portugal
[2] Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England
来源：
BIOTECHNOLOGY ADVANCES | 2005年 / 23卷 / 03期
关键词：
recombinant proteins; Escherichia coli; secretion; periplasm; type II;
D O I：
10.1016/j.biotechadv.2004.11.003
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The secretory production of recombinant proteins by the Gram-negative bacterium Escherichia coli has several advantages over intracellular production as inclusion bodies. In most cases, targeting protein to the periplasmic space or to the culture medium facilitates downstream processing, folding, and in vivo stability, enabling the production of soluble and biologically active proteins at a reduced process cost. This review presents several strategies that can be used for recombinant protein secretion in E. coli and discusses their advantages and limitations depending on the characteristics of the target protein to be produced. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码：177 / 202
页数：26
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