A Single-Crystal EPR Study at 95 GHz of the Type-2 Copper Site of Nitrite Reductase From Alcaligenes faecalis

被引：1
|
作者：
Scarpelli, F. [1 ]
Arrieta, A. L. [2 ]
Gast, P. [1 ]
Groenen, E. J. J. [1 ]
Milikisyants, S.
Murphy, M. E. P. [2 ]
Huber, M. [1 ]
机构：
[1] Leiden Univ, Dept Phys, Huygens Kamerlingh Onnes Lab, NL-2300 RA Leiden, Netherlands
[2] Univ British Columbia, Inst Life Sci, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z3, Canada
来源：
APPLIED MAGNETIC RESONANCE | 2015年 / 46卷 / 04期
基金：
加拿大自然科学与工程研究理事会;
关键词：
ELECTRON-PARAMAGNETIC-RESONANCE; X-RAY-STRUCTURE; PULSED EPR; MUTANT; ENDOR;
D O I：
10.1007/s00723-014-0628-1
中图分类号：
O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];
学科分类号：
070203 ; 070304 ; 081704 ; 1406 ;
摘要：
An electron spin echo-detected electron paramagnetic resonance study has been performed on the type-2 copper site of the nitrite reductase from Alcaligenes faecalis. The experiment on a single crystal at 95 GHz has allowed the determination of the complete g tensor. This includes the orientations of the principal axes of three g tensors in the asymmetric unit of the unit cell with respect to the crystallographic axes. The orientation with respect to the crystallographic axes has been translated into the orientation of the g tensor in the type-2 copper site. The corresponding electronic structure is discussed in relation to the enzymatic function of this copper site.
引用
收藏
页码：411 / 420
页数：10
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