An efficient template-independent polymerase chain displacement reaction for the detection of Salmonella typhimurium

被引：5

作者：

Wang, Jing ^{[1
]}

Li, Haigang ^{[1
]}

Li, Tingting ^{[1
]}

Zhang, Ji ^{[2
]}

Ling, Liansheng ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Sch Chem, Guangzhou 510275, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Canc Ctr, Collaborat Innovat Ctr Canc Med, Dept Neurosurg,State Key Lab Oncol South China, Guangzhou 510060, Guangdong, Peoples R China

来源：

ANALYTICAL METHODS | 2018年 / 10卷 / 35期

基金：

中国国家自然科学基金;

关键词：

ROLLING CIRCLE AMPLIFICATION; REACTION RT-PCR; REAL-TIME PCR; NUCLEIC-ACIDS; ISOTHERMAL AMPLIFICATION; SENSITIVE DETECTION; GOLD NANOPARTICLES; FLUOROGENIC PROBES; MICRORNA DETECTION; COMPLEX;

D O I：

10.1039/c8ay01625a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Here, we incorporated the tailed tandem repeat gene-specific primertechnique into PCDR (TTR-PCDR), developing a template-independent PCDR which allows a rapid, one-tube reaction. Moreover, this TTR-PCDR was applied to the efficient detection of Salmonella typhimurium.

引用

页码：4229 / 4232

页数：4

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