Modulation of Microglial Process Convergence Toward Neuronal Dendrites by Extracellular Calcium

被引：94
作者：
Eyo, Ukpong B. [1 ]
Gu, Nan [1 ,2 ]
De, Srijisnu [1 ]
Dong, Hailong [2 ]
Richardson, Jason R. [3 ]
Wu, Long-Jun [1 ,4 ]
机构：
[1] Rutgers State Univ, Dept Cell Biol & Neurosci, Piscataway, NJ 08854 USA
[2] Fourth Mil Med Univ, Dept Anesthesia, Xijing Hosp, Xian 710032, Peoples R China
[3] Rutgers State Univ, Robert Wood Johnson Med Sch, Dept Environm & Occupat Med, Piscataway, NJ 08854 USA
[4] Anhui Med Univ, Sch Basic Med Sci, Dept Physiol, Hefei 230032, Peoples R China
来源：
JOURNAL OF NEUROSCIENCE | 2015年 / 35卷 / 06期
关键词：
calcium reduction; microglia; microglial process convergence; neuronal dendrites; P2Y12; receptor; GREEN FLUORESCENT PROTEIN; HEMICHANNELS; ACTIVATION; REQUIRES; CA2+;
D O I：
10.1523/JNEUROSCI.3279-14.2015
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Extracellular calcium concentrations in the brain fluctuate during neuronal activities and may affect the behavior of brain cells. Microglia are highly dynamic immune cells of the brain. However, the effects of extracellular calcium concentrations on microglial dynamics have not been investigated. Here, we addressed this question in mouse brain slices and in vivo using two-photon microscopy. We serendipitously found that extracellular calcium reduction induced microglial processes to converge at distinct sites, a phenomenon we termed microglial process convergence (MPCs). Our studies revealed that MPCs target neuronal dendrites independent of neuronal action potential firing and is mediated by ATP release and microglial P2Y12 receptors. These results indicate that microglia monitor and interact with neurons during conditions of cerebral calcium reduction in the normal and diseased brain.
引用
收藏
页码：2417 / 2422
页数：6
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